conjugate
unconjugated
antibody form
purified antibody
clone
34betaE12, monoclonal
purified by
using protein G
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunohistochemistry: suitable, western blot: suitable
isotype
IgG1κ
target post-translational modification
unmodified
species reactivity
mouse
General description
66.04, 62.38, 58.83, and 51.56 kDa calculated for Cytokeratin 1, 5, 10, and 14, respectively.
Solubilized keratin protein derived from human stratum corneum.
Application
Immunohistochemistry (Paraffin) Analysis: A representative lot detected Cytokeratin HMW in Immunohistochemistry applications (Varma, M., et. al. (2003). Histopathology. 42(2):167-72; Gown, A.M., et. al. (1984). Am J Pathol. 114(2):309-21; Cimpean, A.M., et. al. (1999) Rom J Morphol Embryol. 45:153-7).
Western Blotting Analysis: A representative lot detected Cytokeratin HMW in Western Blotting applications (Gown, A.M., et. al. (1984). Am J Pathol. 114(2):309-21).
Western Blotting Analysis: A representative lot detected Cytokeratin HMW in Western Blotting applications (Gown, A.M., et. al. (1984). Am J Pathol. 114(2):309-21).
Biochem/physiol Actions
Clone 34betaE12 is a mouse monoclonal antibody that detects human cytokeratin.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunohistochemistry (Paraffin) in human skin tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Cytokeratin HMW in human skin tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Cytokeratin HMW in human skin tissue sections.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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