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Merck
CN

MABT20

Sigma-Aldrich

Anti-OL-protocadherin Antibody, clone 5G10

clone 5G10, from rat

Synonym(s):

protocadherin 10, ortholog of OL-pcdh

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

rat

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

5G10, monoclonal

species reactivity

mouse

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_001091641

UniProt accession no.

Q925I8

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Pcdh10(18526)

General description

OL-protocadherin (OL-pc, protocadherin 10) is a transmembrane protein and a member of the cadherin superfamily, which is characterized by its members’ abilities to undergo interactions that are homophilic in nature via their extracellular domain. Several protocadherins have been observed within the nervous system, and a few have been shown to localize at synapses. With OL-pc, the homophilic binding has been shown to stimulate cell aggregation when the protein is introduced into cadherin-deficient cells. Although the function of OL-protocadherin has yet to be elucidated, studies have shown OL-pc to be necessary to axonal normal projection through the ventral telencephalon, where OL-pc shows highest expression at the striatal region.
~ 130 kDa observed

Immunogen

Epitope: Cytoplasmic domain
GST-tagged recombinant protein corresponding to the cytoplasmic domain of mouse OL-protocadherin.

Application

Anti-OL-protocadherin Antibody, clone 5G10 is an antibody against OL-protocadherin for use in WB, IC, IH.
Immunofluorescence Analysis: The antibody has been shown to detect OL-protocadherin in IF. (Nakao, 2008).

Immunohistochemistry Analysis: The antibody has been shown to detect OL-protocadherin in IH. (Aoki, 2003).
Research Category
Cell Structure

Neuroscience
Research Sub Category
Adhesion (CAMs)

Synapse & Synaptic Biology

Biochem/physiol Actions

The antibody recognizes the cytoplasmic domain of OL-protocadherin.

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse E13-E14 brain tissue lysate
Evaluated by Western Blot in mouse E13-E14 brain tissue lysate.

Western Blot Analysis: 1 µg/mL of the antibody detected OL-protocadherin in 10 µg of mouse E13-E14 brain tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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