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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5F8.1, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ANO1(55107)
General description
Discovered on gastrointestinal stromal tumors protein 1 (Dog1), also known as Anoctamin-1/TMEM16A, is a novel multi-pass membrane protein belonging to the anoctamin family. Anoctamin-1/TMEM16A functions as a Ca2+-activated Cl- channel and is a required component of tracheal development. Expression of Anoctamin-1/TMEM16A is elevated in the gastrointestinal stromal tumors (GISTs), KIT signaling-driven mesenchymal tumors of the GI tract. It is also expressed at more moderate levels in epithelia from foregut, kidney, lung, pancreas and salivary glands. Recent studies suggest that it may serve a roll as a diagnostic marker in the classification of GISTs.
~114 kDa and ~98 kDa observed. Three isoforms are known to exist at 114 kDa (isoform 1), 98 kDa (isoform 2), and 74 kDa (isoform 3).
Immunogen
Epitope: N-terminal cytoplasmic domain
GST-tagged recombinant protein corresponding to the N-terminal cytoplasmic domain of human Anoctamin-1/TMEM16A.
Application
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Anoctamin-1/TMEM16A in human prostate cancer sections.
This Anti-Anoctamin-1/TMEM16A Antibody, clone 5F8.1 is validated for use in western blotting & IHC for the detection of Anoctamin-1/TMEM16A.
Biochem/physiol Actions
This antibody recognizes isoforms 1 and 2 of Anoctamin-1 based upon immunogen design.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in PC-3 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Anoctamin-1/TMEM16A in 10 µg of PC-3 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Anoctamin-1/TMEM16A in 10 µg of PC-3 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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