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Merck
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MABT64

Anti-Nectin-4/PVRL4 Antibody, clone N4.61

clone N4.61.2, from mouse

Synonym(s):

poliovirus receptor-related 4, Ig superfamily receptor LNIR, nectin 4, poliovirus receptor-related protein 4, nectin-4, Nectin-4

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
N4.61.2, monoclonal
Application:
ELISA
immunohistochemistry
immunoprecipitation (IP)
Species reactivity:
human
Citations:
4
Technique(s):
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
Uniprot accession no.:
Q96NY8

Key Documents

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Product Name

Anti-Nectin-4/PVRL4 Antibody, clone N4.61, clone N4.61.2, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

N4.61.2, monoclonal

species reactivity

human

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1κ

NCBI accession no.

NP_112178

UniProt accession no.

Q96NY8

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... PVRL4(81607)

Immunogen

Recombinant protein corresponding to human Nectin-4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Analysis Note

Control
Ductal carcinoma tissue
Evaluated by Immunohistochemistry in ductal carcinoma tissue.

Immunohistochemistry Analysis: A 1:400 dilution of this antibody detected Nectin-4/PVRL4 in ductal carcinoma tissue.

Application

Anti-Nectin-4/PVRL4 Antibody, clone N4.61 is an antibody against Nectin-4/PVRL4 for use in IH, ELISA & IP.
ELISA Anaysis: A previous lot was used by an independent laboratory in ELISA (Fabre-Lafay, S., et al. (2005). Journal of Biological Chemistry. 280(20):19543–19550).

Immunoprecipitation Anaysis: A previous lot was used by an independent laboratory in IP (Fabre-Lafay, S., et al. (2005). Journal of Biological Chemistry. 280(20):19543–19550).
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

55 kDa calculated
Nectin-4 is also known as poliovirus receptor-related protein 4 (PRR4 or PVRL4). Nectins are known for being the receptors for the herpes simplex virus. Nectin-4 is an immunoglobulin-like transmembrane cell adhesion protein that also seems to play a role in neural tube formation, with N-cadherin. Nectin-4 is connected with the actin cytoskeleton through afadin inside the cell. Nectin-4 is predominantly found in human placenta although in mice, expression is wide-ranging. Nectin-4 seems to adhere primarily to cells expressing nectin-1 and vice versa.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kristin L M Boylan et al.
Oncotarget, 8(6), 9717-9738 (2016-12-31)
The cell adhesion molecule Nectin-4 is overexpressed in epithelial cancers, including ovarian cancer. The objective of this study was to determine the biological significance of Nectin-4 in the adhesion, aggregation, migration, and proliferation of ovarian cancer cells. Nectin-4 and its
Justin W Kaufman et al.
Journal of virology, 97(10), e0105123-e0105123 (2023-09-21)
For many years, measles virus (MeV) was assumed to first enter the host via the apical surface of airway epithelial cells and subsequently spread systemically. We and others reported that MeV has an overwhelming preference for entry at the basolateral

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