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MABT819

Sigma-Aldrich

Anti-Glycosaminoglycan Antibody, skin specific Antibody, clone PG-4

clone PG-4, from mouse

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Synonym(s):
Proteoglycan, Dermatan sulfate, Chondroitin sulfate
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PG-4, monoclonal

species reactivity

human, chicken, shark

packaging

antibody small pack of 25 μL

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgMκ

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... FAM20B(9917)

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antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

PG-4, monoclonal

clone

recombinant monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

human, chicken, shark

species reactivity

human

species reactivity

rat, mouse, monkey, human

species reactivity

rat

packaging

antibody small pack of 25 μL

packaging

antibody small pack of 25 μL

packaging

-

packaging

-

technique(s)

ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable

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General description

Proteoglycans (PG), present in all tissues, are one of the best-studied classes of matrix molecules. Over 50 members of PG have been characterized and studied for their biological significance. PG consist of a protein core to which glycosaminoglycan chains are covalently linked. Glycosaminoglycans (GAGs) are natural heteropolysaccharides that are present in every mammalian tissue. They are composed of repeating disaccharide units that consist of either sulfated or non-sulfated monosaccharides. Their molecular size and the sulfation type vary based on tissue type. The major types of GAGs found in mammalian tissues are: hyaluronan, chondroitin sulfate, and dermatan sulfate, heparin and heparan sulfate, and keratan sulfate. GAG chains are covalently bound to serine residues of the PG protein core via a tetrasaccharide linkage, consisting of xylose, two galactose residues and glucuronic acid. This antibody, clone PG-4, recognizes antigen that is associated with extracellular matrices and surfaces of cultured human dermal fibroblasts and the surfaces of cultured human adult keratinocytes. This antibody recognize native epitopes located within glycosaminoglycan chains and is detects both chondroitin sulfate (Versican) and dermatan sulfate (Decorin and Biglycan) proteoglycans. Treatment of PG with chondroitinases is reported to abolish its immunoreactivity for all antigens. (Ref.: Afratis, N et al. (2012). FEBS Journal 279 (7); 1177-1197; Sorrell, JM et al. (1999). Histochem. J. 31 (8); 549-558).

Specificity

Clone PG4 detects glycosaminoglycan in skin cells.

Immunogen

Crude membrane fractions from adult human dermal fibroblasts from breast skin.

Application

Anti-Glycosaminoglycan, skin specific, clone PG-4, Cat. No. MABT819, is a mouse monoclonal antibody that detects glycosaminoglycan and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
Research Category
Cell Structure
Western Blotting Analysis: A representative lot detected Glycosaminoglycan in Western Blotting applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).

ELISA Analysis: A representative lot detected Glycosaminoglycan in ELISA applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).

Immunohistochemistry Analysis: A representative lot detected Glycosaminoglycan in Immunohistochemistry applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).

Quality

Evaluated by Immunohistochemistry (Paraffin) in human skin tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Glycosaminoglycan in human skin tissue.

Target description

~60 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Mahasish Shome et al.
Methods in molecular biology (Clifton, N.J.), 2597, 131-142 (2022-11-15)
Protein microarrays are an important tool when analyzing multiple analytes simultaneously. As the human genome contains approximately 20,000 genes, examining the interactions of even just one representative protein for each gene requires a high-throughput technique. For instance, the interaction between

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