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About This Item
material
Durapore PVDF membrane
clear acrylic plate
flat bottom wells
Quality Level
description
Non-sterile, clear 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for Avidin-biotin Linkages, DNA-binding Proteins, Protein Binding & PAMPA. Comes in a pack of 50.
with underdrain
sterility
non-sterile
usage
Manual
feature
hydrophobic
lid
packaging
pkg of 50 plates
manufacturer/tradename
MultiScreen®
technique(s)
cell based assay: suitable
dot blot: suitable
parallel artificial membrane permeation assay (PAMPA): suitable
filtration area
0.3 cm2
plate size
96 well
well maximum volume
300 μL
working volume
50-250 μL
matrix
Immobilon®-P
pore size
0.45 μm pore size
binding type
high binding surface
compatibility
manifold only
shipped in
ambient
Application
- as artificial membrane support and a receiver plate in parallel artificial membrane permeation assays (PAMPA) to determine the permeabilities of oximes
- in enzyme-linked immunospot (ELISpot) assay
- in keyhole limpet hemocyanin (KLH)-specific ELISPOT for the identification of antigen (Ag)-specific cells
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Certificates of Analysis (COA)
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Articles
Combining solubility & PAMPA assays streamlines drug permeability testing.
This is a white paper on the Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA) using MultiScreen® Filter Plates.
This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes
Protocols
Assays that predict passive absorption of orally administered drugs have become increasingly important in the drug discovery process. As previously described by Faller and Kansy such assays provide rapid, low cost and automation friendly methods to measure a compound’s passive permeability.
Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.
Related Content
MultiScreen Immobilon-P Filtration Plates
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