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MATFLAGELLIN

NEP Control Flagellin- PyroMAT® System MAT

Non-endotoxin pyrogen control of flagellin for use in monocyte activation test

Synonym(s):

NEP, NEP Control

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About This Item

UNSPSC Code:
41116130
NACRES:
NA.24
eCl@ss:
32160606
Suitability:
pyrogenic bacteria
Manufacturer/tradename:
PyroMAT®
Application(s):
pharmaceutical
pyrogen testing
Storage temp.:
−20°C

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assay

≥95% (SDS-PAGE)

usage

sufficient for 10 tests

feature

Non-endotoxin pyrogen control of flagellin for use in monocyte activation test

manufacturer/tradename

PyroMAT®

application(s)

pharmaceutical
pyrogen testing

compatibility

configured for PyroDetect System, configured for PyroMAT® System

shipped in

cooler

storage temp.

−20°C

suitability

pyrogenic bacteria

Quality Level

400

Related Categories

General description

The PyroMAT® System uses cryopreserved Mono-Mac-6 (MM6) human monocytic cells as a source of monocytes. The response to a full range of pyrogens, including endotoxins and non-endotoxin pyrogens (NEPs)is determined by the measurement of interleukin-6 (IL-6) produced by the Mono-Mac-6 cells using ELISA.
NEP control flagellin is a preparation of flagellin from Salmonella typhimurium and supplied in a lyophilized form. Flagellin is a non-endotoxin pyrogen (NEP) that activates the TLR5 receptor of monocytes and can be used as a positive control with the PyroMAT® System (Monocyte Activation Test for pyrogen detection).

Application

NEP control of flagellin can be used as a positive control with the PyroMAT® System. It is to be used for Product Specific Validation (PSV) and routine testing to show that the system can detect non-endotoxin pyrogen contaminants in pharmaceutical products.

Biochem/physiol Actions

TLR-5 agonist

Features and Benefits

Endotoxin-free product at concentration of use in MAT Purity: ≥ 95% (SDS-PAGE)

Preparation Note

Store unopened vials at -20°C
Do not use unopened vials after expiration date
Store resuspended aliquots at -20°C
Shelf life of aliquots can be found on the technical datasheet

Legal Information

PYROMAT is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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