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MHA000P2M

Millipore

m-Green Yeast and Mold Broth

ready-to-use, ampule of 2 mL, suitable for microbiology, for yeasts, for molds

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Synonym(s):
M-Yeast and Mould Broth, M-BCG (Bromo Cresol Green) Yeast and Mould Broth
eCl@ss:
32160801
NACRES:
NA.74

form

liquid

Quality Level

100

shelf life

12 mo.

feature

ready-to-use

packaging

ampule of 2 mL
pack of 50 tests

color

green

input

sample type cider
sample type food(s)
sample type beer
sample type soft drink
sample type sport drink
sample type cosmetics
sample type beverage(s)
sample type wine
sample type beverage(s)
sample type bottled water

pH

4.6±0.2 (25.0 °C)

application(s)

clinical
cosmetics
environmental
food and beverages
industrial qc
water monitoring
microbiology

compatibility

for use with EZ-Fit® Filtration Manifold
for use with MF-method
for use with MicropreSure®
for use with Milliflex®

shipped in

wet ice

storage temp.

2-8°C

suitability

molds
yeasts

Related Categories

General description

m-Green Yeast and Mold Broth is a relatively more complex formula compared to other media used for the isolation of fungi and yeast. This formulation is rich in nutrients which allows excellent fungal growth. Bacterial growth is inhibited by an acidic pH. Enzymatic digest of casein and enzymatic digest of animal tissue provide nitrogen, carbon, and amino acids in m-Green yeast and mold Broth. Yeast extract is the vitamin source. Dextrose is an energy source for metabolism of fungi. Potassium phosphate is a buffering agent. Magnesium sulphate, Thiamine, and Diastase (a mixture containing amylolytic (starch) enzymes) provide essential ions, minerals, and nutrients. Bromocresol Green is the pH indicator, facilitating visualization and counting of fungal colonies. The colonies are green due to the diffusion of bromocresol green into the colonies (alkaline reaction). Acidic end products from the colonies diffuse into the medium, further reducing the pH and causing the dye to turn yellow (acid reaction). Yeast produces green, opaque large colonies. Mold colonies are green and filamentous.

Application

m-Green yeast and mold Broth is used for the testing soft drinks and other beverages like beer, bottled water, cider, soft drink, sport drink and wine for growth of yeast and mold.

Features and Benefits

  • Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
  • They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.

Specifications

Incubation Temperature: 28–32 °C
Incubation Time: 48–72 hrs

Analysis Note

QC organisms
S. cerevisiae (ATCC 9763)

A.brasilliensisformerly A. niger (ATCC 16404 / WDCM 00053)
Target Microorganisms: Yeast and mold (selection by pH)
Yeast produce green, opaque large colonies. Mold colonies are green and filamentous

Legal Information

EZ-FIT is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Media for Isolation-Identification-Cultivation-Maintenance of Medical Bacteria
MacFaddin JF
Williams and Wilkins (1985)

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