NS170
Multi Species Phosphorylated Neurofilament ELISA Kit
measures and quantifies pNF-H levels in serum or plasma
Synonym(s):
200 kDa neurofilament protein, Phosphorylated Neurofilament heavy polypeptide, pNF-H
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Species reactivity:
pig, feline, rat, human, bovine, mouse
Shipped in:
dry ice
Product Name
Phosphorylated Neurofilament (pNF-H) Sandwich ELISA Kit, The Phosphorylated Neurofilament H (pNF-H) kit is a sandwich enzyme immunoassay (EIA), which measures pNF-H.
Quality Level
species reactivity
pig, feline, rat, human, bovine, mouse
manufacturer/tradename
Chemicon®
assay range
sensitivity: 0.0293-15 ng/mL
(Range of Detection), sensitivity: 0.0585 ng/mL
technique(s)
ELISA: suitable
NCBI accession no.
UniProt accession no.
application(s)
research use
detection method
colorimetric
shipped in
dry ice
Application
Research Category
Neuroscience
Neuroscience
The Phosphorylated Neurofilament H (pNF-H) kit is a sandwich enzyme immunoassay (EIA), which measures pNF-H.
Used to detect/quantify: Phosphorylated Neurofilament (pNF-H)
Packaging
96 wells
Preparation Note
Prior to use maintain the kit components at 2°-8°C and -20°C, as indicated, until expiration date.
1. Wash Buffer - After dilution maintain the Wash Solution at 2°-8°C for up to one month or until expiration date.
2. Tween® 20 - Store at room temperature upon receipt of kit.
3. Elisa Assay Buffer - Aliquot as required to avoid repeated freeze-thaw cycles. After thawing, maintain the Elisa Assay Buffer at 2°-8°C for up to one month, or until expiration date.
4. Immunoplate Pre-coated with Chicken anti- pNF-H Polyclonal Antibody - Unused strip wells may be returned to the foil pouch and stored at 2°-8°C for up to one month, or until expiration date.
1. Wash Buffer - After dilution maintain the Wash Solution at 2°-8°C for up to one month or until expiration date.
2. Tween® 20 - Store at room temperature upon receipt of kit.
3. Elisa Assay Buffer - Aliquot as required to avoid repeated freeze-thaw cycles. After thawing, maintain the Elisa Assay Buffer at 2°-8°C for up to one month, or until expiration date.
4. Immunoplate Pre-coated with Chicken anti- pNF-H Polyclonal Antibody - Unused strip wells may be returned to the foil pouch and stored at 2°-8°C for up to one month, or until expiration date.
Other Notes
Immunoplate Pre-coated with Chicken anti-pNF-H Polyclonal Antibody: (Part No. 2006263). One 96-well immunoplate pre-coated with chicken anti- pNF-H polyclonal antibody sealed in a foil pouch.
Rabbit anti- pNF-H Polyclonal Antibody: (Part No. 2006919). One vial (100 μL) of rabbit anti- pNF-H polyclonal antibody.
Goat anti-Rabbit, Alkaline Phosphatase Conjugated Antibody: (Part No. 2006917). One vial (10 μL) of goat anti-rabbit alkaline phosphatase conjugate.
Bovine pNF-H Standard: (Part No. 2006918). One vial (5.0 ng) of bovine pNF-H standard.
20X TBS: (Part No. 2004913). One bottle (50 mL) of 20X TBS.
20% Tween® 20: (Part No. 2004915). One vial (3 mL) of 20% Tween-20.
Elisa Assay Diluent: (Part No. 2006921) One bottle (30 mL) of Elisa assay diluent containg preservative, provided in a ready to use format.
pNPP Buffer: (Part No. 2006261). One bottle (12 mL) of pNPP buffer for use with 50X p-NPP, provided in a ready to use format.
50X pNPP: (Part No. 2006262). One vial (250 μL) of 50X pNPP (p-nitrophenyl phosphatase) alkaline phosphatase substrate.
Plate Covers - Two Plate Covers.
Rabbit anti- pNF-H Polyclonal Antibody: (Part No. 2006919). One vial (100 μL) of rabbit anti- pNF-H polyclonal antibody.
Goat anti-Rabbit, Alkaline Phosphatase Conjugated Antibody: (Part No. 2006917). One vial (10 μL) of goat anti-rabbit alkaline phosphatase conjugate.
Bovine pNF-H Standard: (Part No. 2006918). One vial (5.0 ng) of bovine pNF-H standard.
20X TBS: (Part No. 2004913). One bottle (50 mL) of 20X TBS.
20% Tween® 20: (Part No. 2004915). One vial (3 mL) of 20% Tween-20.
Elisa Assay Diluent: (Part No. 2006921) One bottle (30 mL) of Elisa assay diluent containg preservative, provided in a ready to use format.
pNPP Buffer: (Part No. 2006261). One bottle (12 mL) of pNPP buffer for use with 50X p-NPP, provided in a ready to use format.
50X pNPP: (Part No. 2006262). One vial (250 μL) of 50X pNPP (p-nitrophenyl phosphatase) alkaline phosphatase substrate.
Plate Covers - Two Plate Covers.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Disclaimer
For research use only. Not for use in diagnostic procedures.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
target_organs
Kidney,Liver,Blood
Storage Class
10 - Combustible liquids
wgk
WGK 3
Regulatory Information
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Gerry Shaw et al.
Biochemical and biophysical research communications, 336(4), 1268-1277 (2005-09-24)
Several lines of reasoning suggest that the phosphorylated axonal form of the neurofilament subunit NF-H is likely to be released from damaged and diseased neurons in significant amounts. Detection of this protein in serum or CSF might therefore provide information
P N Hoffman et al.
The Journal of cell biology, 66(2), 351-366 (1975-08-01)
This study of the slow component of axonal transport was aimed at two problems: the specific identification of polypeptides transported into the axon from the cell body, and the identification of structural polypeptides of the axoplasm. The axonal transport paradigm
Kevin J Anderson et al.
Journal of neurotrauma, 25(9), 1079-1085 (2008-08-30)
The detection of neuron-specific proteins in blood might allow quantification of the degree of neuropathology in experimental and clinical contexts. We have been studying a novel blood biomarker of axonal injury, the heavily phosphorylated axonal form of the high molecular
Global Trade Item Number
| SKU | GTIN |
|---|---|
| NS170 | 04053252010699 |