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OP17L

Anti-c-Fos (Ab-1) Mouse mAb (2G9C3)

lyophilized, clone 2G9C3, Calbiochem®

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
2G9C3, monoclonal
Species reactivity:
rat, human, mouse
Application:
—
Citations:
-

Key Documents

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biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

2G9C3, monoclonal

form

lyophilized

does not contain

preservative

species reactivity

rat, human, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Immunoblotting (2.5-5 µg/mL)
Immunocytochemistry (2.5-5 µg/mL)
Paraffin Sections (not recommended))

isotype

IgG2a

shipped in

ambient

Quality Level

100

Gene Information

human ... FOS(2353)

target post-translational modification

unmodified

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General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with AG.8653X mouse myeloma cells. Recognizes the ~55 kDa c-fos and the ~62 kDa v-fos proteins.
Recognizes both the ~55 kDa c-Fos and ~62 kDa v-Fos proteins in HeLa cells. Does not cross-react with the ~39 kDa Jun protein.
This Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of c-Fos (Ab-1).

Immunogen

Human
a synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos

Application

Immunoblotting (2.5-5 µg/ml)

Immunocytochemistry (2.5-5 µg/ml)

Paraffin Sections (not recommended)

Physical form

Lyophilized from a volatile buffer, 200 µg BSA.

Preparation Note

Reconstitute in sterile PBS, PH 7.4, or sterile Tris-saline (20 mM Tris containing 150 mM NaCl), pH 7.4, to a final concentration of 200 µg/ml. To achieve complete solubilization, reconstitute at (4°C) with 0.1% sodium azide or aliqout and freeze (-20°C) for long-term storage. Note: sodium azide is cytotoxic.

Analysis Note

Positive Control
HeLa cells

Other Notes

Jensen, D.E., et al. 1991. Oncogene6, 1219.
Reiss, M., et al. 1990. Cancer Res.50, 6641.
RouscherIII, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.
Verma, I.M., et al. 1987. Cancer Res.49, 29.
Verma, I.M., et al. 1987. Cell51, 513.
Muller, R. 1986. Viochem. Biophys. Acta.823, 207.
Verma, P. 1986. Trends Genet.2, 93.
Greenberg, M., et al. 1984. Nature (London)311, 433.
Recognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class

11 - Combustible Solids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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