biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
76, monoclonal
form
liquid
does not contain
preservative
species reactivity
human, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
General description
Purified mouse monoclonal antibody. Recognizes the ~32-39 kDa c-Jun/AP-1 protein.
Recognizes the ~39 kDa c-Jun/AP-1 protein in proliferating cells and most tumor tissues.
Immunogen
recombinant jun protein
Application
Immunoblotting (2.5 g/ml)
Paraffin Sections (2.5 g/ml, pressure cooker pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 0.05 M sodium phosphate buffer, 50% glycerol.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
Quiescent cells
Quiescent cells
Positive Control
Growing cells (such as HeLa) or breast or colon tissue
Growing cells (such as HeLa) or breast or colon tissue
Other Notes
This is the preferred c-Jun antibody for immunoblotting. Antibody should be titrated for optimal results in individual systems.
Volm, M., et al. 1993. Clin. Exp. Metastasis11, 325.
Angel, P., et al. 1988. Nature332, 166.
Rouscher III, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Bohmann, D., et al. 1987. .Science238, 1386.
Vogt, P.K., et al. 1987. Proc. Natl. Acad. Sci. USA84, 3316.
Arndt, K., et al. 1986. Proc. Natl. Acad. Sci. USA83, 8516.
Cavaleri, F., et al. 1985. Virology143, 680.
Angel, P., et al. 1988. Nature332, 166.
Rouscher III, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Bohmann, D., et al. 1987. .Science238, 1386.
Vogt, P.K., et al. 1987. Proc. Natl. Acad. Sci. USA84, 3316.
Arndt, K., et al. 1986. Proc. Natl. Acad. Sci. USA83, 8516.
Cavaleri, F., et al. 1985. Virology143, 680.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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