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Merck
CN

PC138

Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb

liquid, Calbiochem®

别名:

Anti-Nuclear Factor ΚB

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关于此项目

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human
Application:
Citations:
4
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biological source

rabbit

Quality Segment

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA
Gel Shift
Immunoblotting (1:500, chemiluminescence)
Immunoprecipitation )

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Rabbit polyclonal antibody. Recognizes the ~65 kDa NF-κB p65 (RelA) protein.
Recognizes the ~65 kDa NF-κB p65 (RelA) protein in Daudi cells.
This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2).

Immunogen

Human
a synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH

Application

ELISA (see comments)

Gel Shift (see comments)

Immunoblotting (1:500, chemiluminescence)

Immunoprecipitation (see comments)

Physical form

In 5 mM sodium phosphate buffer, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Daudi cells

Other Notes

Kolberg, R., 1997. J. NIH Research9, 29.
Van Antwerp, D.J. et al. 1996. Science274, 787.
Beg, A.A., et al. 1995. Nature376, 167.
Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141.
Wulczyn, F.G., et al. 1992. Nature358, 597.
This antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)


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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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