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PC223L

Anti-Neuropeptide Y Rabbit pAb

Name: Anti-Neuropeptide Y Rabbit pAb
Brand: MM

lyophilized, Calbiochem^®

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

polyclonal

Species reactivity:

human, avian, amphibian, fish, feline, rat, newt

Application:

—

Citations:

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biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

contains

≤0.1% sodium azide as preservative

species reactivity

human, avian, amphibian, fish, feline, rat, newt

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

dilution

(Frozen Sections (1:1000-1:2000 for free-floating, PAP detection))

isotype

IgG

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NPY(4852)

General description

Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes neuropeptide Y.

Recognizes neuropeptide Y in cortex, caudate, putamen, and paraventricular nucleus.

This Anti-Neuropeptide Y Rabbit pAb is validated for use in Frozen Sections for the detection of Neuropeptide Y.

Immunogen

porcine neuropeptide Y

Application

Frozen Sections (1:1000-1:2000 for free-floating, PAP detection)

Preparation Note

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Paraventricular nucleus, cortex, or caudate putamen

Other Notes

Antibody specificity was examined in the rat cortex, caudate putamen, and paraventricular nucleus. Staining is completely eliminated by pretreatment of antibody with neuropeptide Y (10^-6M). Preabsorption with avian pancreatic polypeptide, β-endorphin, cholecystokinin, peptide YY, somatostatin, or vasoactive intestinal peptide resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c