PF126
Bcl-2, C-Terminal Truncated, His•Tag®, Human, Recombinant, E. coli
Recombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His•Tag sequence, and expressed in E. coli.
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
biological source
human
Quality Level
recombinant
expressed in E. coli
Assay
≥95% (SDS-PAGE)
form
liquid
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Neutralization (see biological activity))
shipped in
wet ice
storage temp.
−20°C
General description
Recombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His•Tag sequence, and expressed in E. coli. The truncated protein retains the ability to neutralize pro-apoptotic Bcl-2 family members.
Application
Neutralization (see biological activity)
Biochem/physiol Actions
EC₅₀ = 100-300 nM based on its ability to inhibit 54 nM caspase-8-cleaved human Bid-mediated release of cytochrome c from isolated mouse liver mitochondria
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM KCl, 10 mM HEPES, 20% glycerol, pH 8.0.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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