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Merck
CN

PS1008-M

PhosphoDetect Anti-FAK (pTyr⁸⁶¹) Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-FADK1, Phospho-Specific (Tyr⁸⁶¹), Anti-pp125FAK, Phospho-Specific (Tyr⁸⁶¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr⁸⁶¹), Anti-PTK2, Phospho-Specific (Tyr⁸⁶¹), FAK 1

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
mouse, human, chicken
Application:
—
Citations:
-

Key Documents

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biological source

rabbit

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, human, chicken

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

isotype

IgG

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr861)

Quality Level

100

Gene Information

human ... PTK2(5747)

General description

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic phosphotyrosine-containing peptide, followed by immunoaffinity chromatography. Recognizes the ~125 kDa FAK protein phosphorylated at Tyr861.
Recognizes the ~125 kDa FAK protein phosphorylated at Tyr861 in primary chick embryo fibroblasts transfected with human FAK.
This PhosphoDetect Anti-FAK (pTyr⁸⁶¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of FAK (pTyr⁸⁶¹).

Immunogen

a synthetic phosphopeptide surrounding the Tyr⁸⁶¹ phosphorylation site of human FAK

Physical form

In Dulbecco′s PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).

Analysis Note

Positive Control
Chicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin

Other Notes

Eliceiri, B.P., et al. 2002. J. Cell. Biol.157, 149.
Hauck, C.R., et al. 2002. J. Biol. Chem.277, 12487.
Lacalle, R.A., et al. 2002. J. Cell. Biol.157, 277.
Lim, Y., et al. 2002. J. Biol. Chem.277, 12735.
Melendez, J., et al. 2002. J. Biol. Chem.227, 45203.
Rigacci, S., et al. 2002. J. Biol. Chem.277, 41631.
Datta, A., et al. 2001. Mol. Cell. Biol.21, 7295.
Nakamura, K., et al. 2001. Oncogene20, 2626.
Rocic, P., et al. 2001. J. Biol. Chem.276, 21902.
Slack, J.K., et al. 2001. Oncogene20, 1152.
Sieg, D.J., et al. 2000. Nature Cell. Biol.2, 249.
Vial, D., et al. 2000. J. Biol. Chem.275, 28269.
Wennerberg, K., et al 2000. Mol. Cell. Biol.20, 5758.
The immunogen sequence is 100% conserved in rat and Xenopus FAK but cross-reactivity has not been tested. This antibody is also reported to work for immunohistochemistry. Antibody should be titrated for optimal use in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

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Storage Class

10 - Combustible liquids

wgk

WGK 1

Regulatory Information

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