PS1008-M
PhosphoDetect Anti-FAK (pTyr⁸⁶¹) Rabbit pAb
liquid, Calbiochem®
别名:
Anti-FADK1, Phospho-Specific (Tyr⁸⁶¹), Anti-pp125FAK, Phospho-Specific (Tyr⁸⁶¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr⁸⁶¹), Anti-PTK2, Phospho-Specific (Tyr⁸⁶¹), FAK 1
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关于此项目
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
mouse, human, chicken
Application:
—
Citations:
-
biological source
rabbit
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, human, chicken
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr861)
Quality Level
Gene Information
human ... PTK2(5747)
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General description
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic phosphotyrosine-containing peptide, followed by immunoaffinity chromatography. Recognizes the ~125 kDa FAK protein phosphorylated at Tyr861.
Recognizes the ~125 kDa FAK protein phosphorylated at Tyr861 in primary chick embryo fibroblasts transfected with human FAK.
This PhosphoDetect Anti-FAK (pTyr⁸⁶¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of FAK (pTyr⁸⁶¹).
Immunogen
a synthetic phosphopeptide surrounding the Tyr⁸⁶¹ phosphorylation site of human FAK
Physical form
In Dulbecco′s PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).
Analysis Note
Positive Control
Chicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
Chicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
Other Notes
Eliceiri, B.P., et al. 2002. J. Cell. Biol.157, 149.
Hauck, C.R., et al. 2002. J. Biol. Chem.277, 12487.
Lacalle, R.A., et al. 2002. J. Cell. Biol.157, 277.
Lim, Y., et al. 2002. J. Biol. Chem.277, 12735.
Melendez, J., et al. 2002. J. Biol. Chem.227, 45203.
Rigacci, S., et al. 2002. J. Biol. Chem.277, 41631.
Datta, A., et al. 2001. Mol. Cell. Biol.21, 7295.
Nakamura, K., et al. 2001. Oncogene20, 2626.
Rocic, P., et al. 2001. J. Biol. Chem.276, 21902.
Slack, J.K., et al. 2001. Oncogene20, 1152.
Sieg, D.J., et al. 2000. Nature Cell. Biol.2, 249.
Vial, D., et al. 2000. J. Biol. Chem.275, 28269.
Wennerberg, K., et al 2000. Mol. Cell. Biol.20, 5758.
Hauck, C.R., et al. 2002. J. Biol. Chem.277, 12487.
Lacalle, R.A., et al. 2002. J. Cell. Biol.157, 277.
Lim, Y., et al. 2002. J. Biol. Chem.277, 12735.
Melendez, J., et al. 2002. J. Biol. Chem.227, 45203.
Rigacci, S., et al. 2002. J. Biol. Chem.277, 41631.
Datta, A., et al. 2001. Mol. Cell. Biol.21, 7295.
Nakamura, K., et al. 2001. Oncogene20, 2626.
Rocic, P., et al. 2001. J. Biol. Chem.276, 21902.
Slack, J.K., et al. 2001. Oncogene20, 1152.
Sieg, D.J., et al. 2000. Nature Cell. Biol.2, 249.
Vial, D., et al. 2000. J. Biol. Chem.275, 28269.
Wennerberg, K., et al 2000. Mol. Cell. Biol.20, 5758.
The immunogen sequence is 100% conserved in rat and Xenopus FAK but cross-reactivity has not been tested. This antibody is also reported to work for immunohistochemistry. Antibody should be titrated for optimal use in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
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10 - Combustible liquids
wgk
WGK 1
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