PS1008-M
PhosphoDetect Anti-FAK (pTyr⁸⁶¹) Rabbit pAb
liquid, Calbiochem®
别名:
Anti-FADK1, Phospho-Specific (Tyr⁸⁶¹), Anti-pp125FAK, Phospho-Specific (Tyr⁸⁶¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr⁸⁶¹), Anti-PTK2, Phospho-Specific (Tyr⁸⁶¹), FAK 1
生物来源
rabbit
质量水平
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
mouse, human, chicken
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pTyr861)
基因信息
human ... PTK2(5747)
一般描述
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic phosphotyrosine-containing peptide, followed by immunoaffinity chromatography. Recognizes the ~125 kDa FAK protein phosphorylated at Tyr861.
Recognizes the ~125 kDa FAK protein phosphorylated at Tyr861 in primary chick embryo fibroblasts transfected with human FAK.
This PhosphoDetect Anti-FAK (pTyr⁸⁶¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of FAK (pTyr⁸⁶¹).
免疫原
a synthetic phosphopeptide surrounding the Tyr⁸⁶¹ phosphorylation site of human FAK
外形
In Dulbecco′s PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).
分析说明
Positive Control
Chicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
Chicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
其他说明
Eliceiri, B.P., et al. 2002. J. Cell. Biol.157, 149.
Hauck, C.R., et al. 2002. J. Biol. Chem.277, 12487.
Lacalle, R.A., et al. 2002. J. Cell. Biol.157, 277.
Lim, Y., et al. 2002. J. Biol. Chem.277, 12735.
Melendez, J., et al. 2002. J. Biol. Chem.227, 45203.
Rigacci, S., et al. 2002. J. Biol. Chem.277, 41631.
Datta, A., et al. 2001. Mol. Cell. Biol.21, 7295.
Nakamura, K., et al. 2001. Oncogene20, 2626.
Rocic, P., et al. 2001. J. Biol. Chem.276, 21902.
Slack, J.K., et al. 2001. Oncogene20, 1152.
Sieg, D.J., et al. 2000. Nature Cell. Biol.2, 249.
Vial, D., et al. 2000. J. Biol. Chem.275, 28269.
Wennerberg, K., et al 2000. Mol. Cell. Biol.20, 5758.
Hauck, C.R., et al. 2002. J. Biol. Chem.277, 12487.
Lacalle, R.A., et al. 2002. J. Cell. Biol.157, 277.
Lim, Y., et al. 2002. J. Biol. Chem.277, 12735.
Melendez, J., et al. 2002. J. Biol. Chem.227, 45203.
Rigacci, S., et al. 2002. J. Biol. Chem.277, 41631.
Datta, A., et al. 2001. Mol. Cell. Biol.21, 7295.
Nakamura, K., et al. 2001. Oncogene20, 2626.
Rocic, P., et al. 2001. J. Biol. Chem.276, 21902.
Slack, J.K., et al. 2001. Oncogene20, 1152.
Sieg, D.J., et al. 2000. Nature Cell. Biol.2, 249.
Vial, D., et al. 2000. J. Biol. Chem.275, 28269.
Wennerberg, K., et al 2000. Mol. Cell. Biol.20, 5758.
The immunogen sequence is 100% conserved in rat and Xenopus FAK but cross-reactivity has not been tested. This antibody is also reported to work for immunohistochemistry. Antibody should be titrated for optimal use in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Irritant (B)
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
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