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UNSPSC Code:
12352203
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biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr1007/pTyr1008)

Gene Information

human ... JAK2(3717)

General description

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~130 kDa JAK2 protein.
Recognizes the ~130 kDa JAK2 protein phosphorylated at Tyr1007/1008 in LIF-treated 3T3-L1 cells.
This PhosphoDetect Anti-JAK2 (pTyr1007/1008) Rabbit pAb is validated for use in Immunoblotting for the detection of JAK2 (pTyr1007/1008).

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1007/1008 phosphorylation sites of human JAK2

Application


Immunoblotting (1:1000)

Warning

Toxicity: Irritant (B)

Physical form

In Dulbecco′s PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (± 0.1).

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
3T3-L1 adipocytes stimulated with leukemia inhibitor factor (LIF)

Other Notes

Antibody should be titrated for optimal results in individual systems.
Frank, G.D., et al. 2003. Mol. Biol.23, 1581.
Kaszubska, W., et al. 2002. Mol. Cell. Endocrinol.195, 109.
Kirito, K., et al. 2002. J. Biol. Chem.277, 8329.
Shaw, S., et al. 2002. J. Biol. Chem.277, 44920.
Sutherland, C.L., et al. 2002. J. Immunol.168, 671.
Zeng, Z.Z., et al. 2002. J. Biol. Chem.277, 41213.
Eguchi, S., et al. 2001. J. Biol. Chem.276, 7957.
Madamanchi, N.R., et al. 2001. J. Biol. Chem.276, 18915.
Myers, M.P., et al. 2001. J. Biol. Chem.276, 47771.
Madamanchi, N.R., et al. 2000. Arterioscler. Thromb. Vasc. Biol.21, 321.
Amiri, F., et al. 1999. J. Biol. Chem.274, 32382.
Ihle, J.N., et al. 1996. Cell84, 331.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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