PS1014
PhosphoDetect Anti-JAK2 (pTyr1007/1008) Rabbit pAb
liquid, Calbiochem®
别名:
Anti-Janus Kinase 2, Phospho-Specific (Tyr 1007/1008, Anti-Janus Kinase 2, Phospho-Specific (Tyr1007/1008
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关于此项目
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, rat, mouse
Application:
—
Citations:
-
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr1007/pTyr1008)
Quality Level
Gene Information
human ... JAK2(3717)
General description
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~130 kDa JAK2 protein.
Recognizes the ~130 kDa JAK2 protein phosphorylated at Tyr1007/1008 in LIF-treated 3T3-L1 cells.
This PhosphoDetect Anti-JAK2 (pTyr1007/1008) Rabbit pAb is validated for use in Immunoblotting for the detection of JAK2 (pTyr1007/1008).
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1007/1008 phosphorylation sites of human JAK2
Application
Immunoblotting (1:1000)
Physical form
In Dulbecco′s PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (± 0.1).
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
3T3-L1 adipocytes stimulated with leukemia inhibitor factor (LIF)
3T3-L1 adipocytes stimulated with leukemia inhibitor factor (LIF)
Other Notes
Antibody should be titrated for optimal results in individual systems.
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Kaszubska, W., et al. 2002. Mol. Cell. Endocrinol.195, 109.
Kirito, K., et al. 2002. J. Biol. Chem.277, 8329.
Shaw, S., et al. 2002. J. Biol. Chem.277, 44920.
Sutherland, C.L., et al. 2002. J. Immunol.168, 671.
Zeng, Z.Z., et al. 2002. J. Biol. Chem.277, 41213.
Eguchi, S., et al. 2001. J. Biol. Chem.276, 7957.
Madamanchi, N.R., et al. 2001. J. Biol. Chem.276, 18915.
Myers, M.P., et al. 2001. J. Biol. Chem.276, 47771.
Madamanchi, N.R., et al. 2000. Arterioscler. Thromb. Vasc. Biol.21, 321.
Amiri, F., et al. 1999. J. Biol. Chem.274, 32382.
Ihle, J.N., et al. 1996. Cell84, 331.
Kaszubska, W., et al. 2002. Mol. Cell. Endocrinol.195, 109.
Kirito, K., et al. 2002. J. Biol. Chem.277, 8329.
Shaw, S., et al. 2002. J. Biol. Chem.277, 44920.
Sutherland, C.L., et al. 2002. J. Immunol.168, 671.
Zeng, Z.Z., et al. 2002. J. Biol. Chem.277, 41213.
Eguchi, S., et al. 2001. J. Biol. Chem.276, 7957.
Madamanchi, N.R., et al. 2001. J. Biol. Chem.276, 18915.
Myers, M.P., et al. 2001. J. Biol. Chem.276, 47771.
Madamanchi, N.R., et al. 2000. Arterioscler. Thromb. Vasc. Biol.21, 321.
Amiri, F., et al. 1999. J. Biol. Chem.274, 32382.
Ihle, J.N., et al. 1996. Cell84, 331.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
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存储类别
10 - Combustible liquids
wgk
WGK 1
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