生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human, rat, mouse
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pTyr1007/pTyr1008)
基因信息
human ... JAK2(3717)
一般描述
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~130 kDa JAK2 protein.
Recognizes the ~130 kDa JAK2 protein phosphorylated at Tyr1007/1008 in LIF-treated 3T3-L1 cells.
This PhosphoDetect Anti-JAK2 (pTyr1007/1008) Rabbit pAb is validated for use in Immunoblotting for the detection of JAK2 (pTyr1007/1008).
免疫原
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1007/1008 phosphorylation sites of human JAK2
应用

Immunoblotting (1:1000)
警告
Toxicity: Irritant (B)
外形
In Dulbecco′s PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (± 0.1).
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
3T3-L1 adipocytes stimulated with leukemia inhibitor factor (LIF)
3T3-L1 adipocytes stimulated with leukemia inhibitor factor (LIF)
其他说明
Antibody should be titrated for optimal results in individual systems.
Frank, G.D., et al. 2003. Mol. Biol.23, 1581.
Kaszubska, W., et al. 2002. Mol. Cell. Endocrinol.195, 109.
Kirito, K., et al. 2002. J. Biol. Chem.277, 8329.
Shaw, S., et al. 2002. J. Biol. Chem.277, 44920.
Sutherland, C.L., et al. 2002. J. Immunol.168, 671.
Zeng, Z.Z., et al. 2002. J. Biol. Chem.277, 41213.
Eguchi, S., et al. 2001. J. Biol. Chem.276, 7957.
Madamanchi, N.R., et al. 2001. J. Biol. Chem.276, 18915.
Myers, M.P., et al. 2001. J. Biol. Chem.276, 47771.
Madamanchi, N.R., et al. 2000. Arterioscler. Thromb. Vasc. Biol.21, 321.
Amiri, F., et al. 1999. J. Biol. Chem.274, 32382.
Ihle, J.N., et al. 1996. Cell84, 331.
Kaszubska, W., et al. 2002. Mol. Cell. Endocrinol.195, 109.
Kirito, K., et al. 2002. J. Biol. Chem.277, 8329.
Shaw, S., et al. 2002. J. Biol. Chem.277, 44920.
Sutherland, C.L., et al. 2002. J. Immunol.168, 671.
Zeng, Z.Z., et al. 2002. J. Biol. Chem.277, 41213.
Eguchi, S., et al. 2001. J. Biol. Chem.276, 7957.
Madamanchi, N.R., et al. 2001. J. Biol. Chem.276, 18915.
Myers, M.P., et al. 2001. J. Biol. Chem.276, 47771.
Madamanchi, N.R., et al. 2000. Arterioscler. Thromb. Vasc. Biol.21, 321.
Amiri, F., et al. 1999. J. Biol. Chem.274, 32382.
Ihle, J.N., et al. 1996. Cell84, 331.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
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