QIA88
ProteoExtract® Cytosol/Mitochondria Fractionation Kit
Synonym(s):
Cellular Fractionation Kit
usage
sufficient for 100 extractions
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
technique(s)
DNA extraction: suitable, RNA extraction: suitable, nuclear extraction: suitable, protein extraction: suitable
input
sample type cells
shipped in
dry ice
storage temp.
−20°C
General description
Note: 1 Test = reagents sufficient for processing 5 x 107 cells.
This kit provides a unique combination of reagents useful for the isolation of a highly enriched mitochondrial fraction from the cytosol. The enriched fractions can be used to study factors of interest using Western blotting, ELISA, or other assays.
Biochem/physiol Actions
The ProteoExtract Cytosol/Mitochondria Fractionation Kit is designed for extraction of mammalian cells to yield enriched mitochondrial and cytosolic fractions for use in downstream analyses, such as Western blotting.
Preparation Note
• Protease Inhibitor Cocktail: Reconstitute the lyophilized Protease Inhibitor Cocktail by adding 250 µl DMSO; mix well before use. Dispense into aliquots and store at -20°C.• 1X Cytosol Extraction Buffer Mix: Prepare 1X Cytosol Extraction Buffer by diluting the Cytosol Extraction Buffer 1:4 with ddH2O. Prepare only the amount needed for the assay (5 x 107 cells require 1 ml 1X Cytosol Extraction Buffer Mix). Just prior to use add 2 µl Protease Inhibitor Cocktail and 1 µl DTT to 1 ml 1X Cytosol Extraction Buffer.• Mitochondria Extraction Buffer Mix: Prepare only the amount needed for the assay (5 x 107 pelleted cells require 100 µl Mitochondria Extraction Buffer Mix). Just prior to use, add 2 µl Protease Inhibitor Cocktail and 1 µl DTT to 1 ml of Mitochondria Extraction Buffer.
Upon arrival store the entire contents of the kit at -20°C. Following initial use, store the buffers at 4°C and the DTT and Protease Inhibitor Cocktail at -20°C.
Other Notes
Mitochondria Extraction Buffer, Cytosol Extraction Buffer, Protease Inhibitor Cocktail, Reducing Reagent, and a user protocol.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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