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Merck
CN

S2EM004M99

Multiscreen® 96 well Plate, hydrophobic PVDF membrane

pore size 0.45 μm, sterile

Synonym(s):

Hydrophobic 96-Well Plate, PVDF 96-Well Plate

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About This Item

UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32039006
Material:
Durapore PVDF membrane, acrylic plate
Pore size:
0.45 μm pore size
Sterility:
sterile
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Quality Level

material

Durapore PVDF membrane, acrylic plate

description

Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

sterility

sterile

usage

Manual

packaging

pkg of 10 plates

manufacturer/tradename

MultiScreen®

technique(s)

ELISPOT: suitable, cell based assay: suitable

filtration area

0.28 cm2

plate size

96 wells

working volume

50-250 μL

matrix

Immobilon®-P

pore size

0.45 μm pore size

compatibility

manifold only

shipped in

ambient

storage temp.

10-30°C

General description

Filter Code: IP

Application

MultiScreen®-IP Filter Plate, 0.45 μm, white, sterile has been used in the enzyme-linked immunospot (ELISPOT) assay.

Physical form

Color Code: White

Other Notes

Automation Compatible: No

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany


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Protocols

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

Articles

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

Related Content

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. The following protocol is an example of a typical ELISpot assay for quantifying the number cells producing interferon-gamma (IFN-γ) in response to antigen or non-specific activation using phytohemagglutinin (PHA). It may be optimized as necessary for other applications.


Sung-Ho Chang et al.
Cells, 9(5) (2020-05-24)
We investigated the immunogenicity of allogeneic human adipose-derived mesenchymal stem cells (ADSCs) through the production of alloreactive-CD8 T and -memory CD8 T cells, based on their human leukocyte antigen (HLA) expression. In surface antigen analysis, ADSCs do not express co-stimulatory
Sarah Côté-Bigras et al.
Clinical endocrinology, 80(2), 296-300 (2013-06-06)
While thyrotrophin receptor (TSHR) is recognized as the main autoantigen in Graves' disease (GD), the actual antigen specificity of T cells that infiltrate the thyroid and the orbit is unknown. Identifying T cell responses to TSHR peptides has been difficult
Daniela Frasca et al.
Vaccine, 34(25), 2834-2840 (2016-04-25)
The success of a vaccine in inducing a protective antibody response depends on the longevity of both long-lived plasma cells (PC) and memory B cells. We have previously shown that the in vivo antibody response to a new influenza vaccine



Global Trade Item Number

SKUGTIN
S2EM004M9904053252667800