SAE176-100UG
SAvPhire Monomeric Streptavidin Alpha Fluor 488
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
conjugate
horse radish peroxidase (HRP)
Quality Level
clone
monoclonal
species reactivity
all
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
General description
15.5 kDa for SAvPhire Monomeric Streptavidin (with an N-terminal His-tag and a C-terminal FLAG tag).
Upon reconstitution in 100 μL of distilled water the concentration will be 1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Application
Epitope: Unknown
Biochem/physiol Actions
SAvPhire Monomeric Streptavidin expressed in E. coli and conjugated with Alpha Fluor 488 that is suitable for immunodetection of biotinylated proteins.
Physical form
SAvPhire Monomeric Streptavidin Alpha Fluor 488 lyophilized from PBS containing D-Mannitol and Sucrose without azide.
Preparation Note
Stable for 1 year at +2°C to +8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in A431 cells.
Immunofluorescent analysis of A431 cells performed using a 1:1 mixture of biotinylated Anti-EGFR (1:1,000 dilution) and SAvPhire Monomeric Streptavidin Alpha Fluor 488 detected EGFR.
Immunofluorescent analysis of A431 cells performed using a 1:1 mixture of biotinylated Anti-EGFR (1:1,000 dilution) and SAvPhire Monomeric Streptavidin Alpha Fluor 488 detected EGFR.
Other Notes
Predicted to react with all species.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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