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Merck
CN

SAE176-100UG

SAvPhire Monomeric Streptavidin Alpha Fluor 488

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UNSPSC Code:
12352203
NACRES:
NA.46
eCl@ss:
32160702
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conjugate

horse radish peroxidase (HRP)

clone

monoclonal

species reactivity

all

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable

Quality Level

General description

15.5 kDa for SAvPhire Monomeric Streptavidin (with an N-terminal His-tag and a C-terminal FLAG tag).
Upon reconstitution in 100 μL of distilled water the concentration will be 1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Application

Epitope: Unknown

Biochem/physiol Actions

SAvPhire Monomeric Streptavidin expressed in E. coli and conjugated with Alpha Fluor 488 that is suitable for immunodetection of biotinylated proteins.

Physical form

SAvPhire Monomeric Streptavidin Alpha Fluor 488 lyophilized from PBS containing D-Mannitol and Sucrose without azide.

Preparation Note

Stable for 1 year at +2°C to +8°C from date of receipt.

Analysis Note

Evaluated by Immunocytochemistry in A431 cells.



Immunofluorescent analysis of A431 cells performed using a 1:1 mixture of biotinylated Anti-EGFR (1:1,000 dilution) and SAvPhire Monomeric Streptavidin Alpha Fluor 488 detected EGFR.

Other Notes

Predicted to react with all species.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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