THP-1 NF-kappaB-eGFP Human Acute Monocytic Leukemia Cell Line

Microbial contamination of biological cultures is a universal concern for laboratories. Detection and monitoring of microbial contamination often require a significant commitment of time, labor and expense, especially when maintaining multiple cultures and preserving precious specimens. Methods that are at once efficient, cost-effective, and sensitive for a range of microbial contamination are of great value in facilitating detection and ensuring contamination-free cultures.

The THP-1 NF-κB-eGFP cell line makes use of the toll-like receptor (TLR) signaling pathway as a simple and sensitive biological assay for detection of microbial contamination. The suite of TLR proteins recognize distinct molecular signatures conserved across bacterial species and activate the transcription factor NF-κB. THP-1 NF-κB-eGFP cells exhibit high sensitivity for mycoplasma and gram-negative bacterial LPS (1). Incubation of THP-1 NF-κB-eGFP cells for 24 hours with contaminated culture supernatants activates expression of the eGFP reporter which may be easily detected via flow cytometry or light microscopy (1). Presence of amphotericin B in cultures also induces the reporter gene.

Source:
The THP-1 NF-κB-eGFP cell line is derived from a single-cell clone of THP-1 cells stably transfected with an NF-κB-eGFP reporter construct (1). The parental THP-1 cell line was derived from peripheral blood of a 1-year-old male suffering from acute monocytic leukemia (2).

References:
1. PLOS One. 2017; 12(5): e0178220
2. Int J Cancer. 1980; 26(2): 171-176.

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Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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