hERG1a/1b Inducible HEK293 Cell Line

HERG channel inhibition assays for preclinical cardiac safety screening of new drug compounds use electorphysiological methodology and employ hERG-expressing recombinant cell lines. Despite evidence that the cardiac IKr potassium current is produced by heteromeric hERG1a/1b channels1, 2, 3, drug screening assays have long relied on homomeric hERG1a alone, because of the instability of hERG 1b expression in so-called “stable” recombinant cell lines4. The novel inducible hERG1a/1b heteromeric cell line offers an improved tool for preclinical cardiac toxicity screening, by enabling more accurate predictions of off-target drug effects on cardiac IKr, particularly in computational models relying on accurate IC50 measurements. hERG1a/1b cells also can facilitate the study of heteromeric assembly of the hERG 1a and 1b subunits during biogenesis.5

HERG channel inhibition assays for preclinical cardiac safety screening of new drug compounds use electorphysiological methodology and employ hERG-expressing recombinant cell lines. Despite evidence that the cardiac IKr potassium current is produced by heteromeric hERG1a/1b channels1, 2, 3, drug screening assays have long relied on homomeric hERG1a alone, because of the instability of hERG 1b expression in so-called “stable” recombinant cell lines4. The novel inducible hERG1a/1b heteromeric cell line offers an improved tool for preclinical cardiac toxicity screening, by enabling more accurate predictions of off-target drug effects on cardiac IKr, particularly in computational models relying on accurate IC50 measurements. hERG1a/1b cells also can facilitate the study of heteromeric assembly of the hERG 1a and 1b subunits during biogenesis.5 Source The heteromeric hERG1a/1b Inducible HEK293 cell line, in which hERG1b protein expression is tightly controlled by doxycyclin dose and treatment time, was derived from a homomeric hERG 1a expressing HEK293 cell line.5 References1. J Biol Chem 2004, 279(43): 44690–446942. Circ Res 2008, 103(7): e81–e953. PNAS 2014, 111(50): 18073-180774. Br J Pharmacol 2011, 164(2b): 419-432. 5. Biophysical J 2018, Volume 114, Issue 3, Suppl 1, 2 (292a) (Poster Abstract)

• Each vial contains ≥ 1X10⁶ viable cells.• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.• Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services• Cells are negative for mycoplasma contamination.

The heteromeric hERG1a/1b Inducible HEK293 cell line, in which hERG1b protein expression is tightly controlled by doxycyclin dose and treatment time, was derived from a homomeric hERG 1a expressing HEK293 cell line

The cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting functionality.

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