OSUMMER.4 Mouse NRAS-Mutant Melanoma Cell Line

Each vial contains >1X10⁶ viable cells.

Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel and have been found to be negative for all pathologies in this test.

Cells are verified to be of mouse origin and negative for interspecies contamination from rat, human, Chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination Clear panel.

Cells are negative for mycoplasma contamination.

Cells are genotyped by STR analysis to verify the unique identity of the cell line.

The OSUMMER (Ohio State University and Moffitt Melanoma Exposed to Radiation) cell lines fulfill a previously unfilled gap in melanoma biology. These cell lines are syngeneic with C57BL/6 laboratory mice and have genetic profiles that are similar to those of human tumors, making them responsive to immunotherapy treatments. These NRAS-mutant cell lines can be used to discover and address potential immunotherapies in NRAS-mutant human melanomas, which make up an estimated 15-30% of human melanomas. These cell lines also enable in vivo testing of immunotherapies with a mouse model.

OSUMMER.6 NRAS mouse melanoma cell line was derived from a male TN mouse (homozygous for the LSL-NrasQ61R allele) exposed to UVA and UVB radiation. OSUMMER.4 can form tumors in vivo without Matrigel injection. WES (whole exome sequencing) revealed high SNV (Single Nucleotide variants) and low CNA (copy number alterations) burden, as well as the presence of UV signature mutations. OSUMMER.4 cells express the melanoma markers PMEL and MelanA and exhibit robust growth in culture. OSUMMER.6 cells demonstrate a relatively low (<lt/>200) mutation burden compared with other OSUMMER NRAS Q61R mutants, and are resistant to MEK inhibition (MEKi).

Source
OSUMMER.6 was derived from melanoma induced in TN (Tyr::CreER) mice exposed to UVA or UVB radiation on postnatal day 3 (1).

References
1. Bowman, R. L., et al. (2021). UVB mutagenesis differs in Nras-and Braf-mutant mouse models of melanoma. Life Science Alliance, 4(9).

The OSUMMER.4 melanoma cell line carries the NRAS muation Q61R and may be used to create syngeneic mouse melanoma models.

Cells should be stored in liquid nitrogen. Cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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