OSUMMER.5 Mouse NRAS-Mutant Melanoma Cell Line

Each vial contains >=1X10⁶ viable cells.

Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel and have been found to be negative for all pathologies in this test.

Cells are verified to be of mouse origin and negative for interspecies contamination from rat, human, Chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination Clear panel.

Cells are negative for mycoplasma contamination.

Cells are genotyped by STR analysis to verify the unique identity of the cell line.

Melanoma is a skin cancer that develops in pigment-producing melanocytes. Sporadic genetic mutations have been associated with melanomas. Immunotherapy treatments targeting these genes or pathways have been proven to have significant benefits in melanoma patients by improving general response and survival.The OSUMMER (Ohio State University and Moffitt Melanoma Exposed to Radiation)cell lines fulfill a previously unfilled gap in melanoma biology. They are derived from the TN mouse model of melanoma, which are homozygous for the LSL-Nras Q61R allele. Oncogene expression is activated by tamoxifen-inducible Cre recombinase in melanocytes. Loss of function is an early event observed in the majority of human melanomas. TN mice additionally carry p16INK4A conditional knockout alleles. OSUMMER.5 NRAS mouse melanoma cell line was derived from a male TN mouse exposed to UVA and UVB radiation. OSUMMER.5 carries the specific NRAS mutation Q61R.WES revealed high SNV (Single Nucleotide variants) and low CNA (Copy Number Alterations) burden, as well as the presence of UV signature mutations. OSUMMER.5 cells express the melanoma markers PMEL and MelanA, and exhibit robust growth in culture.SourceOSUMMER.5 was derived from melanoma induced in TN (Tyr::CreER) mice exposed to UVA or UVB radiation on postnatal day 3.References1. Nat Rev Dis Primers 2015, 1: 150032.The Lancet, 392(10151), 971-984.3. Life Sci Alliance 2021, 4(9): e202101135.

OSUMMER.5 male mouse NRAS-mutant melanoma cell line carries the TN61R mutation and is derived from the TN mouse model. This model is syngeneic with the C57BL/6 mouse model. OSUMMER cells can form tumors in vivo without Matrigel injection.

OSUMMER.5 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.