ReNcell Neural Stem Cell Freezing Medium

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

ReNcell® VM and ReNcell® CX are two well-established neural stem cell lines derived from developing human brains. ReNcell® VM and CX cells are generated from the ventral mesencephalon and cortical regions of the brain, respectively, and transduced with the myc transcription factor. Both cell lines offer phenotype and genotype stability, in addition to the multipotential neuronal differentiation capacity, over long-term culture. This article describes the characteristics and differentiation of ReNcell® lines. Results of calcium and membrane potential changes in response to various ligands are also shown. The convenience to maintain in culture and flexibility to differentiate to individual scientists’ needs make ReNcell® lines the ideal platform for research and discovery.