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SCM082

ES2N Complete Medium Kit

The ES2N Complete medium is a defined serum-free formulation that has been optimized for the differentiation of mouse embryonic stem (ES) & induced pluripotent stem (iPS) cells into neurons.

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
eCl@ss:
32161000
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Quality Level

300

form

liquid

technique(s)

cell culture | stem cell: suitable

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type neural stem cell(s)

shipped in

dry ice

storage temp.

-10 to -25°C

General description

ES2N Complete medium is a defined serum-free formulation that has been optimized for the differentiation of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells into neurons. When used in conjucture with ESGRO Complete Plus medium mouse ES and iPS cells readily differentiate into neurons in a monolayer assay within 9-12 days on gelatin coated culture dishes. When supplemented with bFGF and EGF, this medium enables the isolation and expansion of neural stem cells (NSCs).

Application

The ES2N Complete medium is a defined serum-free formulation that has been optimized for the differentiation of mouse embryonic stem (ES) & induced pluripotent stem (iPS) cells into neurons.

Analysis Note

Cell Proliferation Assay: The biological activity of this lot of VEGF was determined by its mitogenic activity on human umbilical vein endothelial cells (HUVEC) with an ED50 of about 1.0 ng/mL. Results may vary depending on the cell line used. VEGF will also stimulate endothelial cells from other species.

Other Notes

ES2N Basal Medium: (Cat. No. SCM083) One (1) 245 mL bottle containing standard base medium.

Neuro27 Medium Supplement: (Cat. No. SCM013-S) One (1) 5 mL vial containing neural supplement (use as 50x).

Neuro2 Medium Supplement: (Cat. No. SCM012-S) One (1) 1.25 mL vial containing N2-like supplement (use as 200x).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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GHS07

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Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

Regulatory Information

新产品

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Murine Embryonic Stem Cell Culture Procedures & Protocols

The cell culture protocols described in this instructional manual include the in vitro culture of murine ES cells using EmbryoMax products along with ESGRO® mLIF medium supplement, as well as feeder-free and serum-free ES cell culture using the ESGRO Complete™ line of products. Also included in this instructional manual are: methods for serum-free neuronal differentiation of mouse ES cells, iPS cell generation using STEMCCA™ lentivirus kits, cre-recombinase mediated excision of STEMCCA™ genes from iPS cells, derivation and rescue of new and existing ES cell lines using RESGRO™ Culture Medium and ESGRO Complete™ system.

ES2N COMPLETE MEDIUM KIT

Global Trade Item Number

SKUGTIN
SCM08204053252699887

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