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Merck
CN

SCMF002-S

Sigma-Aldrich

FibroGRO® LS Supplement Kit

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About This Item

UNSPSC Code:
12352200
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form

frozen liquid

Quality Level

storage temp.

−20°C

General description

To form FibroGRO®-LS Complete Media Kit both components arerequired: basal medium (Cat# SCMF-BM) and supplement mix (Cat# SCMF002-S).

Application

FibroGRO® Media formulations provide an optimal cell cultureenvironment for many types of human fibroblasts and mouse embryonic fibroblastsThese low-serum/serum-free formulations have been shown to grow these cells atrates that meet or exceed commercially available serum containing media, whilemaintaining excellent cell morphology. The media are packaged in a speciallydesigned UV protective shrinkwrap for added stability, which includes atemperature gauge for added convenience. FibroGRO® Media contain no antimicrobialsand no phenol red, components that can cause cell stress and masking effectsthat may influence experimental results.
FibroGRO®-LS Medium is optimized for low-serum (2%)culture of human fibroblasts and supports the growth of these cells in a 2%serum environment at rates equal to or greater than comparable mediasupplemented with much higher concentrations of FBS. May also be used for theculture of stromal cells, mesenchymal cells and mouse embryonic fibroblasts.FibroGRO®-LS Medium contains no antimicrobials and no phenol red; componentsthat can cause cell stress and “masking effects” that may influenceexperimental results (these are not needed, or recommended, to achieve optimalcell performance). FibroGRO® provides a perfect cell system to establish lowserum human feeder layers for stem cell culture, or as a model to study woundhealing, toxicology or basic cell biology.

Preparation Note

Basal Medium (SCMF-BM) should be stored at 2 - 8ºC. Thespecial UV protective packaging protects FibroGRO Medium from light damage,however users should take care to protect medium from extended exposure tolight. Supplement Kits (SCMF001-S and SCMF002-S) should be stored at -20ºC. Seeindividual labels for expiry date. Supplemented medium should be stored at 2 -8ºC in the dark. Use within 1 month of supplementation. Do not freeze.

Other Notes

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

FIBROGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Regulatory Information

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