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Millipore

Millipore® Steriflip® Vacuum Tube Top Filter

pore size 20.0 μm, Nylon membrane (net), funnel capacity 50 mL, pack of 25 ea

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Synonym(s):
Steriflip® Nylon Net Sterile Centrifuge Tube Top Filter Unit, tube top sterile filter unit, tube top vacuum filter, tube top vacuum sterile filtering unit
eCl@ss:
32031602
NACRES:
NB.24

material

Nylon membrane (net)
acrylic housing
polypropylene

Quality Level

400

sterility

sterile

product line

Steriflip®

feature

holdup volume 0.6 mL
hydrophilic

packaging

pack of 25 ea

parameter

45 °C max. temp.

technique(s)

sterile filtration: suitable

H

138 mm

capacity

50 mL

diam.

6.4 cm

filter diam.

4 cm

filtration area

7 cm2

funnel capacity

50 mL

pore size

20.0 μm pore size

fitting

double lead thread inlet (with vacuum port)
 double lead thread outlet for 50 mL centrifuge tube

shipped in

ambient

Related Categories

General description

The Steriflip® filter unit is designed to work with standard 50 mL centrifuge tubes, which eliminates sample transfer. It is useful for sterilization of aqueous solutions (up to 50 ml), sterile filtration of small batches of culture media, sterile filtration of buffers with high-value protein or small molecule components, and cell isolation.

Application

For Research Use Only

Steriflip® Sterile Centrifuge Tube Top Filter Unit has been used:

  • in cell loading and image acquisition
  • in the isolation and culture of retinal ganglion cells (RGCs) and amacrine cells (ACs) from adult mouse retinas
  • for the isolation of tumors cells post digestion

Features and Benefits

  • Eliminates messy sample transfer via a simple vacuum-assisted, closed system
  • Can isolate cells from cellular debris using any of the various pore-sized nylon net filters
  • Ideal for sterilizing small batches of culture media
  • Attaches to any standard 50 mL centrifuge tube, eliminating unnecessary materials, time, and risk of spillage

Legal Information

Millipore is a registered trademark of Merck KGaA, Darmstadt, Germany
Steriflip is a registered trademark of Merck KGaA, Darmstadt, Germany

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Stephen K Jones et al.
Bio-protocol, 8(7) (2018-05-18)
Whole-lifespan single-cell analysis has greatly increased our understanding of fundamental cellular processes such as cellular aging. To observe individual cells across their entire lifespan, all progeny must be removed from the growth medium, typically via manual microdissection. However, manual microdissection
Bing Guo et al.
Animal models and experimental medicine, 1(2), 134-142 (2019-03-21)
Colorectal cancer (CRC) is the third most commonly diagnosed cancer in males and the second in females worldwide in 2012. In the past 20 years, strong evidence suggests that cancer stem cells are the main culprit of cancer metastasis, chemotherapy resistance
Yong H Park et al.
PloS one, 15(12), e0242426-e0242426 (2020-12-08)
Cell culture is widely utilized to study the cellular and molecular biology of different neuronal cell populations. Current techniques to study enriched neurons in vitro are primarily limited to embryonic/neonatal animals and induced pluripotent stem cells (iPSCs). Although the use

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