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SCR004

Alkaline Phosphatase Detection Kit

This Alkaline Phosphatase Detection Kit is a specific & sensitive tool for the phenotypic assessment of Embryonic Stem (ES) cell differentiation by the determination of AP activity.

Synonym(s):

ALP Stain, Alkaline Phosphtase Stem Cell Staining Kit, Stem Cell AP Staining Kit

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.84
eCl@ss:
32161000

Key Documents

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Product Name

Alkaline Phosphatase Detection Kit, This Alkaline Phosphatase Detection Kit is a specific & sensitive tool for the phenotypic assessment of Embryonic Stem (ES) cell differentiation by the determination of AP activity.

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type cardiac stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)

shipped in

wet ice

Quality Level

100

Related Categories

Application

Embryonic stem (ES) cells are totipotent cells derived from the inner cell mass (ICM) of preimplantation mammalian embryos and are capable of unlimited, undifferentiated proliferation in vitro [1,2,3]. Undifferentiated murine ES cells can be maintained in vitro for extended periods in media containing the cytokine, leukemia-inhibitory factor (LIF) or Millipore′s proprietary ES cell culture reagent, ESGRO [4,5]. The undifferentiated state of ES cells can be characterized by high level of expression of Alkaline Phosphatase (AP) [6], the expression of surface markers including SSEA and TRA antigens and the transcription factor Oct-4.

Millipore′s Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.

Available separately from Millipore are the monoclonal antibodies TRA-2-49 (Catalog Number MAB4349) and TRA-2-54 (Catalog Number MAB4354), which permit the detection of Liver / Bone / Kidney isozyme of AP as expressed by ES cells. When used in flow cytometry, these reagents permit a quantitative assessment of AP expression by ES cells [7].

For Research Use Only; Not for use in diagnostic procedures
Research Category
Stem Cell Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Millipore′s Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.

Other Notes

Fast Red Violet solution (0.8g/L stock) (Part No. 90239). Two 15mL bottles.

Napthol AS-BI phosphate solution (2mg/mL) in a buffer, pH 8.5 (Part No. CS235583). Two bottles.

Preparation Note

The Alkaline Phosphatase Detection Kit consists of two components used for determining AP activity.

When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Carc. 2 - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

Certificates of Analysis (COA)

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Feeder-independent culture of mouse embryonic stem cells using vitamin A/retinol.
Jaspal S Khillan,Liguo Chen
Methods in Molecular Biology null
Efficient feeder-free episomal reprogramming with small molecules.
Junying Yu,Kevin Fongching Chau,Maxim A Vodyanik,Jinlan Jiang,Yong Jiang
Testing null
The ROCK inhibitor Y-27632 enhances the survival rate of human embryonic stem cells following cryopreservation.
Xiangyun Li, Guoliang Meng, Roman Krawetz, Shiying Liu, Derrick E Rancourt
Stem Cells and Development null
A novel method for generating xeno-free human feeder cells for human embryonic stem cell culture.
Guoliang Meng, Shiying Liu, Roman Krawetz, Michael Chan, Judy Chernos, Derrick E Rancourt
Stem Cells and Development null
Theofano Panayiotou et al.
PLoS pathogens, 16(4), e1008468-e1008468 (2020-04-17)
Octamer binding transcription factor-4 (Oct4), is highly expressed in stem cells and has indispensable roles in pluripotency and cellular reprogramming. In contrast to other factors used for cellular reprogramming, a role for Oct4 outside embryonic stem cells has been elusive
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Protocols

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Induced Pluripotent Stem Cell Culture Protocols

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

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A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
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Millipore’s new STEMCCA lentivirus reprogramming kits make it easier than ever to generate induced pluripotent stem (iPS) cells. Unlike traditional iPS generation which requires simultaneous co-infection by four separate expression vectors, the STEMCCA kits use a single polycistronic lentiviral vector to improve efficiency and reduce the number of viral integrations. The STEMCCA vector is comprised of the transcription factors Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences 1,2. It is also available with flanking LoxP sites incorporated for Cre-mediated excision of the exogenous reprogramming transgenes. STEMCCA Vector Advantages: (1) Efficient: uses a single vector with four transcription factors rather than co-transducing four separate expression vectors (2) Minimizes viral integrations: single vector reduces the risks of insertional mutagenesis and viral reactivation and (3) Excisable: Cre/LoxP-regulated version enables removal of reprogramming transgenes.

Collagenase Type I for Enzymatic Passaging of Human Embryonic Stem Cells in HEScGRO Medium
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