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Merck
CN

SCR059

Cardiomyocyte Characterization Kit

The Cardiomyocyte Characterization Kit contains antibodies & controls for the immunocytochemical characterization of cardiomyocytes.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000

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manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable
immunocytochemistry: suitable
western blot: suitable

input

sample type cardiac stem cell(s)
sample type mesenchymal stem cell(s)

shipped in

dry ice

General description

Cardiomyocytes are striated heart muscle cells that possess the unique ability to "self-contract" without central nervous system innervation. The inherent contractile activity of cardiomyocytes serves a critical function to pump blood throughout the circulatory system. Loss or dysfunction of cardiomyocytes due to hypertension (high blood pressure) or myocardial infarction (heart attacks), resulting in ineffective pumping of the heart, is a major cause of death and disabilities in the United States. Recent evidence suggests that embryonic and adult stem cells can be directed to become cardiomyocytes and will provide researchers with new strategies to restore heart function and develop new cardiovascular therapies in the future. Millipore′s Cardiomyocyte Characterization Kit contains a collection of validated antibodies that are specific to cardiomyocytes along with control IgG antibodies. Included in the kit are antibodies that are critical to the structure (actinin and desmin), contractile function (tropomyosin and troponin) and homeostatic control (ANP) of cardiac myocytes to a variety of signals that increase blood pressure. The kit enables researchers to characterize cardiomyocytes in culture using basic immunocytochemistry techniques.
The Cardiomyocyte Characterization Kit contains antibodies and controls for the immunocytochemical characterization of cardiomyocytes. The Cardiomyocyte Characterization Kit may be used on human, mouse and rat cells.

Application

The Cardiomyocyte Characterization Kit contains antibodies & controls for the immunocytochemical characterization of cardiomyocytes.

Preparation Note

Antibodies to Troponin I, Desmin and Sheep IgG are to be stored at 2 to 8C. Antibodies to Tropomyosin, Actinin, Atrial Natriuretic Peptide, Mouse IgG, and Rabbit IgG are to be stored at -20C

Other Notes

20 uL of Tropomyosin, sheep polyclonal (Part No. AB5441-20UL)

50 ug of Troponin I, mouse monoclonal (Part No. MAB1691-50UG)

25 uL of Actinin, mouse monoclonal (Part No. MAB1682-25UL)

25 ug of Atrial Natriuretic Peptide (ANP), rabbit polyclonal (Part No. AB5490-25UG)

40 ug of Desmin, mouse monoclonal (Part No. MAB3430)

100 ug of Mouse control IgG (Part No. PP54-100UG)

100 ug of Rabbit control IgG (Part No. PP64-100UG)

100 ug of Sheep control IgG (Part No. PP441-100UG)

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Related Content

Cellutions Newsletter: 2008, Volume 3
Cellutions Newsletter: 2008, Volume 1
Isolation of Cardiac Stem Cells and their Differentiation into Cardiomyocytes

The isolation and expansion of cardiac stem cells opens new opportunities to the feld of cardiac regenerative medicine. CSCs have recently been isolated from human and murine tissues based upon cell biomarkers (Sca-1, TERT, c-Kit, side population), demonstrating the presence of a non-circulating stem cell niche within the myocardium that is estimated to account for 11-14% of the total cell population1. These cells appear to be bi-potent in their capacity to form cardiomyocytes and vascular endothelial cells. Moreover, preliminary engraftment studies suggest that these cells are ideal candidates for future research on cardiac regeneration. However, the rarity of CSCs coupled with the complex isolation procedure is a signifcant challenge for the advancement of the field. o overcome this obstacle, we have developed an easy-to-use cell isolation kit capable of obtaining a high yield, pure population of CSCs. This advancement allows investigators to obtain a signifcantly greater number of CSCs for their studies without the need for time consuming, complex protocols and expensive cell sorting equipment.

Cellutions Newsletter: 2007, Volume 1
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