BioTracker^™ FarRed Mito Superox Probe

The BioTracker Far Red Mitochondrial Superoxide Live Cell Probe detects superoxide, a reactive oxygen species (ROS) produced in mitochondria by electrons leaking during redox reactions. Cellular control of ROS is important because they are associated with disease, cellular damage and senescence. SCT278 is oxidized by mitochondrial superoxide and accumulated in mitochondria.

The BioTracker Far Red Mitochondrial Superoxide Live Cell Probe SCT278
*enables detection of mitochondrial superoxide with a long wavelength (far red)
*permits co-staining with green or red fluorescent probes
*demonstrates high selectivity for superoxide

SPECTRAL PROPERTIES:
Ex 540 nm
Em 670 nm

Add 10 μL of DMSO to each tube containing SCT278 (100 nmol) to create a 10 mM stock solution. Dilute stock solution with culture medium to create a 10 uM working solution.

BioTracker Far Red Mitochondrial Superoxide Live Cell Probe selectively detects superoxide. and is sensitive enough for small samples. SCT278 fluoresces in a longer wavelength to enhance understanding of how mitochondria are altered during diverse physi

Cell Based Assays Fluorescence Flow Cytometry Fluorescence Activated Cell Sorting (FACS)

Live cell probe for the detection of mitochondrial superoxide, which can alter mitochondrial membrane permeability to promote apoptosis.

Store at 2-8°C. Protect from light.

White to pale purple solid

Mitochondria are important organelles that uses oxygen to synthesize ATP, producing the necessary energy for living cells to thrive. Increased mitochondrial activity and mitochondrial dysfunction are associated with cancer, aging, and neurodegenerative diseases such as Alzheimer′s and Parkinson′s diseases. Mitochondrial mass (MM), mitochondrial membrane potential (MMP), and mitochondrial ROS (mtROS) have been widely studied as promising targets for mitochondria-related diseases. Since these mitochondrial attributes are dynamic, simultaneous analysis using multiple staining in a single sample confers a significant advantage.

Other red fluorescent reagents have widely been used to detect mitochondrial superoxide. However, their emission wavelength is a shorter red, which overlaps with other membrane potential detection probes such as TMRE, and is therefore not applicable for simultaneous staining with other mitochondrial markers in a multiplexed sample.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany