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SLM-240

RPMI-1640 complete medium

With ʟ-glutamine and 10% US origin FBS, liquid, 0.2 μm filtered, suitable for cell culture

Synonym(s):

Low Bicarb Formulation, Roswell Park Memorial Institute 1640 medium

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.71
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Product Name

RPMI 1640 Complete Medium, with 2mM L-Glut and 10% FBS, supplemented with 2mM L-Glutamine and 10% U.S. origin FBS

Quality Level

300

form

liquid

technique(s)

cell culture | mammalian: suitable

General description

RPMI 1640 CompleteMedium is supplemented with 2mM L-Glutamine and 10% U.S.-origin fetal bovine serum (FBS). This solution is convenient to culture a wide range ofcells that grow in suspension or as anchorage dependent. It can also be used infusion protocols and for growing hybrid cells. Roswell Park Memorial Institute(RPMI) 1640 medium can be used for culturing human normal and neoplasticleukocytes. It supports the growth of various cultured cells, like fresh humanlymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay if theformulation is properly supplemented.

Application

RPMI 1640 CompleteMedium, with 2mM L-Glut and 10% FBS has been used to culture: Jurkatlymphocytes and human U937 monocyte-like cell line(1), HT-29 and SW480 (humancolon cancer cell line) cells(2), monocytes(3)

Analysis Note

pH= 7.2-7.5
Osmolality= 270-292 mOsm/kg
Endotoxin= ≤2 EU/mL
Mycoplasma= Negative
Sterility Tested= Negative for Growth
Appearance= Orange/Red Clear Liquid
Cell Line Tested= A2780 Human Ovarian Carcinoma

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Articles

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Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.

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Yimei Jiang et al.
BMC molecular and cell biology, 21(1), 48-48 (2020-07-01)
Colorectal cancer (CRC) is a leading cancer and a major cause of death. Lipopolysaccharide (LPS), an abundant component in gut microbiome, is involved in CRC progression and metastasis, potentially through regulating the miRNA composition of CRC-derived exosomes. In this study
Cristina C Clement et al.
Nature communications, 12(1), 4447-4447 (2021-07-23)
Tryptophan catabolism is a major metabolic pathway utilized by several professional and non-professional antigen presenting cells to maintain immunological tolerance. Here we report that 3-hydroxy-L-kynurenamine (3-HKA) is a biogenic amine produced via an alternative pathway of tryptophan metabolism. In vitro

Global Trade Item Number

SKUGTIN
SLM-240-B04054839387074