ST1027
Anti-Acetylated Lysine Rabbit pAb
liquid, Calbiochem®
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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
-
Application:
—
Citations:
17
Quality Level
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA (1:1000)
Immunoblotting (1:1000)
Immunoprecipitation (1:100)
Paraffin Sections (1:50))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
acetylation (Lys)
General description
Anti-Acetylated Lysine, rabbit polyclonal antibody that recognizes acetylated lysine in UV-treated HEK293 cells. It is validated for ELISA, Western blotting, immunoprecipitation, & paraffin sections.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes acetylated lysine-containing proteins.
Recognizes acetylated lysine residues in UV-treated HEK293 cells.
Immunogen
synthetic acetylated-lysine
Application
ELISA (1:1000)
Immunoblotting (1:1000)
Immunoprecipitation (1:100)
Paraffin Sections (1:50)
Analysis Note
Positive Control
HEK293 cells treated with UV
HEK293 cells treated with UV
Other Notes
Yu, X., et al. 2002. J. Natl. Cancer Inst.94, 504.
Mal, A., et al. 2001. EMBO J.20, 1739.
Sano, Y. and Ishii, S. 2001. J. Biol. Chem.276, 3674.
Mal, A., et al. 2001. EMBO J.20, 1739.
Sano, Y. and Ishii, S. 2001. J. Biol. Chem.276, 3674.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
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Ling Cai et al.
Cell reports, 4(6), 1063-1071 (2013-09-17)
Ribosome biogenesis requires an enormous commitment of energy and resources in growing cells. In budding yeast, the transcriptional coactivator Ifh1p is an essential regulator of ribosomal protein (RP) gene transcription. Here, we report that Ifh1p is dynamically acetylated and phosphorylated
Chao Yang et al.
The Journal of biological chemistry, 287(42), 34917-34926 (2012-08-25)
The MYST family of histone acetyltransferases (HATs) plays critical roles in diverse cellular processes, such as the epigenetic regulation of gene expression. Lysine autoacetylation of the MYST HATs has recently received considerable attention. Nonetheless, the mechanism and function of the
Chao Yang et al.
PloS one, 7(3), e32886-e32886 (2012-04-04)
The 60-kDa HIV-Tat interactive protein (Tip60) is a key member of the MYST family of histone acetyltransferases (HATs) that plays critical roles in multiple cellular processes. We report here that Tip60 undergoes autoacetylation at several lysine residues, including a key
Rtt101-Mms1-Mms22 coordinates replication-coupled sister chromatid cohesion and nucleosome assembly.
Jingjing Zhang et al.
EMBO reports, 18(8), 1294-1305 (2017-06-16)
Two sister chromatids must be held together by a cohesion process from their synthesis during S phase to segregation in anaphase. Despite its pivotal role in accurate chromosome segregation, how cohesion is established remains elusive. Here, we demonstrate that yeast
Frederic Beckouët et al.
Molecular cell, 39(5), 689-699 (2010-09-14)
Sister chromatid cohesion is thought to involve entrapment of sister DNAs by a tripartite ring composed of the cohesin subunits Smc1, Smc3, and Scc1. Establishment of cohesion during S phase depends on acetylation of Smc3's nucleotide-binding domain (NBD) by the
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