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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
4A8, monoclonal
Species reactivity:
human
Application:
—
Citations:
1
Quality Level
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4A8, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Paraffin Sections (3 µg/mL))
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... VCP(7415)
General description
Purified mouse monoclonal antibody. Recognizes the ~67 kDa VCP protein.
Recognizes the ~67 kDa VCP protein in HePG2 cells and human placenta tissue.
This Anti-VCP Mouse mAb (4A8) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of VCP.
Immunogen
Full-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein
Application


ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HePG2 cells, human placenta tissue
HePG2 cells, human placenta tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
新产品
This item has
Certificates of Analysis (COA)
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Philipp Voisard et al.
International journal of molecular sciences, 23(12) (2022-06-25)
Valosin-containing protein (VCP) acts as a key regulator of cellular protein homeostasis by coordinating protein turnover and quality control. Mutations in VCP lead to (cardio-)myopathy and neurodegenerative diseases such as inclusion body myopathy with Paget's disease of the bone and
Global Trade Item Number
| SKU | GTIN |
|---|---|
| ST1553-100UG | 04055977224276 |