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ST1630

Sigma-Aldrich

Anti-FGF8 Mouse mAb (2A10)

liquid, clone 2A10, Calbiochem®

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Synonym(s):
Anti-Fibroblast Growth Factor 8

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

2A10, monoclonal

form

liquid

does not contain

preservative

species reactivity

mouse, human, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Fgf8(14179)

General description

Anti-FGF8, mouse monoclonal, clone 2A10, recognizes the ~30-35 kDa FGF8 protein in PC-12, Jurkat, HepG2, NIH3T3, and Raw 264.7 cells. It is validated for use in ELISA and Western blotting.
Purified mouse monoclonal antibody. Recognizes the ~30-35 kDa FGF8 protein.
Recognizes the ~30-35 kDa FGF8 protein in PC-12, Jurkat, HepG2, NIH3T3, and Raw 264.7 cells.

Immunogen

Full-length, recombinant, human FGF8 (aa 65-133), expressed as a GST fusion protein

Application





ELISA (see comments)
Immunoblotting (1-5 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
PC-12 cells, Jurkat cells, HepG2 cells, NIH3T3 cells, Raw 264.7 cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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