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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
2G1, monoclonal
Species reactivity:
human
Application:
—
Citations:
4
biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
2G1, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Paraffin Sections (0.8 µg/mL))
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PDK2(5164)
General description
Purified mouse monoclonal antibody. Recognizes the ~40 kDa PDK2 protein.
Recognizes the ~40 kDa PDK2 protein in U-2 OS cells.
This Anti-PDK2 Mouse mAb (2G1) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of PDK2.
Immunogen
A recombinant polypeptide corresponding to amino acids of 187-276 human PDK2, expressed as a GST fusion protein
Application


ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (0.8 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
293T cells
Positive Control
U-2 OS cells
U-2 OS cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| ST1643-100UG | 04055977223781 |