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ST1673

Anti-AFP Mouse mAb (1G7)

Name: Anti-AFP Mouse mAb (1G7)
Brand: MM

liquid, clone 1G7, Calbiochem^®

Synonym(s):

Anti-α-Fetoprotein

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

1G7, monoclonal

Species reactivity:

human

Application:

—

Citations:

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Properties

biological source

mouse

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1G7, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA (see coments)
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AFP(174)

Description

General description

Anti-AFP, mouse monoclonal, clone 1G7, recognizes ~70-75 kDa AFP protein in HepG2 cells. It is validated for ELISA, Western blotting, and immunocytochemistry.

Purified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein.

Recognizes the ~70-75 kDa AFP protein in HepG2 cells.

Immunogen

A recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein

Application

ELISA (see coments)

Immunoblotting (1-5 µg/ml)

Immunocytochemistry (10 µg/ml)

Physical form

In PBS, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells

Positive Control
HepG2 cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c