ST1673
Anti-AFP Mouse mAb (1G7)
liquid, clone 1G7, Calbiochem®
Synonym(s):
Anti-α-Fetoprotein
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
1G7, monoclonal
Species reactivity:
human
Application:
—
Citations:
10
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
1G7, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA (see coments)
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... AFP(174)
General description
Anti-AFP, mouse monoclonal, clone 1G7, recognizes ~70-75 kDa AFP protein in HepG2 cells. It is validated for ELISA, Western blotting, and immunocytochemistry.
Purified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein.
Recognizes the ~70-75 kDa AFP protein in HepG2 cells.
Immunogen
A recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein
Application
ELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Physical form
In PBS, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
293T cells
Positive Control
HepG2 cells
HepG2 cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Raymond C B Wong et al.
Stem cell research, 22, 13-15 (2017-09-28)
We report the generation of the hiPSC line CERAi001-A-6 from primary human dermal fibroblasts. Reprogramming was performed using episomal vector delivery of OCT4, SOX2, KLF4, L-MYC, LIN28 and shRNA for p53.
Maciej Daniszewski et al.
iScience, 7, 30-39 (2018-09-30)
We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with
Jenna Hall et al.
SLAS technology (2023-09-02)
Age-Related Macular Degeneration (AMD) is a highly prevalent form of retinal disease amongst Western communities over 50 years of age. A hallmark of AMD pathogenesis is the accumulation of drusen underneath the retinal pigment epithelium (RPE), a biological process also
Global Trade Item Number
| SKU | GTIN |
|---|---|
| ST1673-100UG | 04055977223842 |