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TMS-008

Sigma-Aldrich

Alizarin-Red Staining Solution

Alizarin red is a commonly used stain to identify calcium containing osteocytes in differentiated culture of both human and rodent mesenchymal stem cells (MSCs).

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Synonym(s):
Alizarin Red S, Alizarin Red S Stain, Alizarin Red Stain
NACRES:
NA.75

Quality Level

100

concentration

40 mM in distilled water

technique(s)

cell based assay: suitable
cell culture | stem cell: suitable
cell differentiation: suitable

Related Categories

General description

Alizarin Red, an anthraquinone derivative, may be used to identify calcium in tissue sections and cultured cells in vitro. The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Calcium forms an Alizarin Red S-calcium complex in a chelation process, and the end product is a bright red stain. Alizarin red is a commonly used stain to identify calcium containing osteocyte in differentiated culture of both human and rodent mesenchymal stem cells.

Application

Research Category
Stem Cell Research
Research Sub Category
Osteobiology

Arthritis

Quality

Solution is red with no particulates present. Solution is suitable for endpoint cell culture assays.

Physical form

Product is presented as a 40mM solution at pH 4.1 +/- 0.1.

Storage and Stability

Store at 2-8ºC for up to 4 months from date of receipt. If Alizarin-red solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Protocols

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

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