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About This Item
NACRES:
NB.22
UNSPSC Code:
41105339
Product Name
PVDF Western Blotting Membranes, roll W × L 30 cm × 3.00 m, roll of 1, pore size 0.2 μm
Quality Level
material
PVDF
white PVDF membrane (hydrophobic)
packaging
roll of 1
manufacturer/tradename
Roche
roll W × L
30 cm × 3.00 m
pore size
0.2 μm pore size
capacity
131 μg/cm2 (BSA)
294 μg/cm2 (Goat IgG)
85 μg/cm2 (Insulin)
shipped in
ambient
storage temp.
room temp
Related Categories
Analysis Note
Each lot is function tested (dot blot).
Application
PVDF Western Blotting Membranes has been used in western blot, for transferring proteins for sequencing.
Note: Tested for use with Lumi-Light western blotting substrate or Lumi-LightPlus western blotting substrate.
Note: Tested for use with Lumi-Light western blotting substrate or Lumi-LightPlus western blotting substrate.
- Dot/slot blotting
- Blotting from 2D gels
- Protein sequencing
- HIV detection
- Hybridoma screening
- Cell blotting
- High binding capacity, high mechanical strength, and chemical resistance of the PVDF membrane makes itespecially useful for protein blotting.
- The combination of strong sample retention with low background binding generates excellent signal-to-noise ratioswith chromogenic and radioactive detection techniques.
- The membrane is also ideal for chemiluminescent substrates.
General description
PVDF Western Blotting Membranes are hydrophobic, microporous, polyvinylidene difluoride (PVDF) membranes with a pore size of 0.2μm. The PVDF Western Blotting Membranes are ideal for western and dot blots. The high binding capacity, high mechanical strength, and chemical resistance of the PVDF membrane makes it specifically useful for protein blotting. The combination of strong sample retention and low background binding generates excellent signal-to-noise ratios in colorimetric and chemiluminescent detection. The primary binding mechanism includes electrostatic and hydrophobic nature.
Adsorption: Goat IgG: 294μg/cm2; BSA: 131μg/cm2; insulin: 85μg/cm2
Adsorption: Goat IgG: 294μg/cm2; BSA: 131μg/cm2; insulin: 85μg/cm2
Other Notes
For life science research only. Not for use in diagnostic procedures.
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