material
PVDF , white PVDF membrane (hydrophobic)
packaging
roll of 1
manufacturer/tradename
Roche
roll W × L
30 cm × 3.00 m
pore size
0.2 μm pore size
capacity
131 μg/cm2 (BSA), 294 μg/cm2 (Goat IgG), 85 μg/cm2 (Insulin)
shipped in
ambient
storage temp.
room temp
General description
PVDF免疫印迹膜是疏水性的聚偏二氟乙烯(PVDF)微孔膜,孔径为0.2μm。PVDF免疫印迹膜适用于蛋白质免疫印迹和斑点杂交印迹。PVDF膜具有高结合能力、高机械强度和耐化学腐蚀性,因此,特别适用于蛋白质印迹。强大的样品保留性和低背景结合,使其在比色和化学发光检测中具有出色的信噪比。主要结合原理包括静电吸附和疏水作用。
吸附:山羊IgG:294μg/cm2;BSA:131μg/cm2;胰岛素:85μg/cm2
吸附:山羊IgG:294μg/cm2;BSA:131μg/cm2;胰岛素:85μg/cm2
Application
PVDF免疫印迹膜已用于免疫印迹,转印蛋白质以供测序使用。
注意:经测试可与Lumi-Light免疫印迹化学发光底物或Lumi-LightPlus免疫印迹化学发光底物一起使用。
注意:经测试可与Lumi-Light免疫印迹化学发光底物或Lumi-LightPlus免疫印迹化学发光底物一起使用。
- Dot/slot blotting
- Blotting from 2D gels
- Protein sequencing
- HIV detection
- Hybridoma screening
- Cell blotting
- High binding capacity, high mechanical strength, and chemical resistance of the PVDF membrane makes itespecially useful for protein blotting.
- The combination of strong sample retention with low background binding generates excellent signal-to-noise ratioswith chromogenic and radioactive detection techniques.
- The membrane is also ideal for chemiluminescent substrates.
Analysis Note
每一批次都经过功能测试(斑点印迹杂交)。
Other Notes
仅用于生命科学研究。不可用于诊断。