产品名称
PVDF免疫印迹膜, roll W × L 30 cm × 3.00 m, roll of 1, pore size 0.2 μm
Quality Level
material
PVDF
white PVDF membrane (hydrophobic)
packaging
roll of 1
manufacturer/tradename
Roche
roll W × L
30 cm × 3.00 m
pore size
0.2 μm pore size
capacity
131 μg/cm2 (BSA)
294 μg/cm2 (Goat IgG)
85 μg/cm2 (Insulin)
shipped in
ambient
storage temp.
room temp
Other Notes
仅用于生命科学研究。不可用于诊断。
Analysis Note
每一批次都经过功能测试（斑点印迹杂交）。
Application
PVDF免疫印迹膜已用于免疫印迹，转印蛋白质以供测序使用。
注意：经测试可与Lumi-Light免疫印迹化学发光底物或Lumi-LightPlus免疫印迹化学发光底物一起使用。
注意：经测试可与Lumi-Light免疫印迹化学发光底物或Lumi-LightPlus免疫印迹化学发光底物一起使用。
- Dot/slot blotting
- Blotting from 2D gels
- Protein sequencing
- HIV detection
- Hybridoma screening
- Cell blotting
- High binding capacity, high mechanical strength, and chemical resistance of the PVDF membrane makes itespecially useful for protein blotting.
- The combination of strong sample retention with low background binding generates excellent signal-to-noise ratioswith chromogenic and radioactive detection techniques.
- The membrane is also ideal for chemiluminescent substrates.
General description
PVDF免疫印迹膜是疏水性的聚偏二氟乙烯(PVDF)微孔膜，孔径为0.2μm。PVDF免疫印迹膜适用于蛋白质免疫印迹和斑点杂交印迹。PVDF膜具有高结合能力、高机械强度和耐化学腐蚀性，因此，特别适用于蛋白质印迹。强大的样品保留性和低背景结合，使其在比色和化学发光检测中具有出色的信噪比。主要结合原理包括静电吸附和疏水作用。
吸附：山羊IgG：294μg/cm2；BSA：131μg/cm2；胰岛素：85μg/cm2
吸附：山羊IgG：294μg/cm2；BSA：131μg/cm2；胰岛素：85μg/cm2
Changcun Zhang et al.
Oncology letters, 7(5), 1689-1694 (2014-04-26)
MicroRNA-34a (miR-34a) is a tumor suppressive microRNA, which induces G1 arrest, apoptosis and senescence by repressing the expression of multiple oncogenes. This study aimed to investigate the biological function and molecular mechanisms of miR-34a in human renal cell carcinoma (RCC)
R M Vejborg et al.
Journal of applied microbiology, 105(1), 141-150 (2008-02-27)
We have recently found that preconditioning of stainless steel surfaces with an aqueous fish muscle extract can significantly impede bacterial adhesion. The purpose of this study was to identify and characterize the primary components associated with this bacteria-repelling effect. The
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Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.
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