biological source
bovine (calf) intestine
form
solution
specific activity
~200 units/mg protein (At 25 °C with adenosine as the substrate.)
mol wt
230-440 kDa (high), 30-47 kDa (low )
packaging
pkg of 2 mL (10 mg)
manufacturer/tradename
Roche
optimum pH
7.0-7.4
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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General description
Adenosine Deaminase (ADA) is present majorly in the proximal small intestine and the duodenum. It is also called Adenosine aminohydrolase. It is a zinc metalloenzyme and exists as a high (230−440 kDa) and low molecular weight (30−47 kDa) isoforms.
Adenosine deaminase (ADA) is a zinc metalloenzyme.
Application
Adenosine Deaminase (ADA) has been used as a component of macrophage- serum-free media (SFM) to treat macrophages.
Adenosine deaminase has been used to pre-treat primary Kupffer cells.
Biochem/physiol Actions
Adenosine Deaminase (ADA) catalyzes the deamination of adenosine analogs to the corresponding inosine analogs. It is present in high levels in colorectal cancer and is a potential marker in tuberculous pleuriti. ADA deficiency affects T-and B-lymphocytes resulting in severe combined immunodeficiency (SCID) leading to lymphocytopenia.
Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.
Physical form
Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6
Preparation Note
Storage conditions (working solution): ADA (all preparations) may be dialyzed, without loss of activity, at 2 to 8 °C against 20 mM phosphate buffer, pH 7. The dialyzed preparation is stable at -15 to -25 °C.
Analysis Note
Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Regulatory Information
低风险生物材料
动植物源性产品
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Adenosine deaminase deficiency: a review
Flinn AM and Gennery AR
Orphanet Journal of Rare Diseases, 13(1), 65-65 (2018)
Pdx-1 is required for activation in vivo from a duodenum-specific enhancer
Dusing MR, et al.
The Journal of biological chemistry, 276(17), 14434-14442 (2001)
Purification and characterization of intestinal adenosine deaminase from mice
Singh L and Sharma R
Molecular and Cellular Biochemistry, 204(1-2), 127-134 (2000)
Development of adenosine deaminase-specific IgY antibodies: Diagnostic and inhibitory application
Lupicka-Slowik A, et al.
Applied Biochemistry and Biotechnology, 184(4), 1358-1374 (2018)
Mi-Jeong Lee et al.
Methods in enzymology, 538, 49-65 (2014-02-18)
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