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10108626001

Roche

Poly(A)

Name: Poly(A)
Brand: ROCHE

lyophilized, suitable for PCR, pkg of 100 mg

Synonym(s):

Poly(A), Polyadenylic acid

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About This Item

UNSPSC Code:

41106305

NACRES:

NA.54

Form:

lyophilized

Solubility:

water: soluble

Storage temp.:

2-8°C

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Properties

description

Polyadenylic acid

Quality Level

100

form

lyophilized

mol wt

700-3500 kDa

packaging

pkg of 100 mg

manufacturer/tradename

Roche

concentration

0.5 mg/mL (Working concentration)

technique(s)

PCR: suitable

color

white

solubility

water: soluble

absorbance ratio

A_290/260 nm 0.03-0.05, A_280/260 nm 0.28-0.32, A_250/260 nm 0.86-0.90

storage temp.

2-8°C

Description

General description

Poly(A) is used as a carrier for quantitative precipitation of DNA and RNA.

Application

Polyadenylic acid (Poly(A)) to inhibit Exo1′s exonuclease activity. It has also been used as a carrier to resuspend lyophilized gBlocks.

Poly(A) has been used for droplet digital PCR (ddPCR) assay.

Biochem/physiol Actions

Polyadenylic acid (Poly(A)) tails present at 3′ end are produced in the cell nucleus. It contains ~250 nucleotides in mammalian cells. Poly(A) regulates mRNA decay and the initiation of translation. Cytoplasmic poly(A) extension modulates translation.

Physical form

Lyophilizate, potassium salt

Preparation Note

Working concentration: 0.5 mg/ml
A concentration of 0.5 mg/ml is recommended.
Working solution: 0.5 mg/ml is recommended.

Analysis Note

Typical analysis:
2.3μmol/mg Poly(A) (from absorbance) in relation to one mononucleotide unit. Chromatographically homogeneous.
Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀

Other Notes

1 A₂₆₀ unit corresponds to 40 μg ssRNA.

Chain Length 2.100 to 10.000 nucleotides

For life science research only. Not for use in diagnostic procedures.

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Poly (A) tail length is controlled by the nuclear poly (A)-binding protein regulating the interaction between poly (A) polymerase and the cleavage and polyadenylation specificity factor

Kuhn U, et al.

The Journal of Biological Chemistry, 284(34), 22803-22814 (2009)

Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing.

Yuichiro Miyaoka et al.

Scientific reports, 6, 23549-23549 (2016-04-01)

Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-directed repair (HDR). However, nonhomologous end-joining (NHEJ), an error-prone repair, acts concurrently, reducing the rate of high-fidelity edits. The identification of genome-editing conditions that

Poly (ADP-ribose)-binding promotes Exo1 damage recruitment and suppresses its nuclease activities

Cheruiyot A, et al.

DNA Repair, 35, 106-115 (2015)

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Global Trade Item Number

SKU	GTIN
10108626001	04061837684432