description
Polyadenylic acid
Quality Level
form
lyophilized
mol wt
700-3500 kDa
packaging
pkg of 100 mg
manufacturer/tradename
Roche
concentration
0.5 mg/mL (Working concentration)
technique(s)
PCR: suitable
color
white
solubility
water: soluble
absorbance ratio
A290/260 nm 0.03-0.05, A280/260 nm 0.28-0.32, A250/260 nm 0.86-0.90
storage temp.
2-8°C
General description
Poly（A）被用作DNA和RNA定量沉淀的载体。
Application
聚腺苷酸Poly(A)抑制 Exo1的核酸外切酶活性。还用作重悬冻干 gBlocks 的载体。
Poly（A）已可用于微滴式数字PCR（ddPCR）分析。
Biochem/physiol Actions
聚腺苷酸(Poly(A))尾部有3′末端，在细胞核产生。哺乳动物细胞中，约含 250 个核苷酸。Poly(A)可调控mRNA降解和翻译起始。胞质Poly(A)的延伸调控翻译过程。
Physical form
冻干品，钾盐
Preparation Note
工作浓度：0.5 mg/ml
建议浓度为0.5 mg/ml。
工作溶液：建议浓度为0.5 mg/ml。
建议浓度为0.5 mg/ml。
工作溶液：建议浓度为0.5 mg/ml。
Analysis Note
典型分析：
2.3μmol/mg Poly(A)（来自吸光度）相当于一个单核苷酸单位。色谱均匀。
吸光度测定 A250/A260、A280/A260、A290/A260
2.3μmol/mg Poly(A)（来自吸光度）相当于一个单核苷酸单位。色谱均匀。
吸光度测定 A250/A260、A280/A260、A290/A260
Other Notes
1个A260 单位相当于40 μg ssRNA。
仅用于生命科学研究。不可用于诊断。
链长为 2.100 至 10.000 个核苷酸
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Poly (A) tail length is controlled by the nuclear poly (A)-binding protein regulating the interaction between poly (A) polymerase and the cleavage and polyadenylation specificity factor
Kuhn U, et al.
The Journal of Biological Chemistry, 284(34), 22803-22814 (2009)
Yuichiro Miyaoka et al.
Scientific reports, 6, 23549-23549 (2016-04-01)
Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-directed repair (HDR). However, nonhomologous end-joining (NHEJ), an error-prone repair, acts concurrently, reducing the rate of high-fidelity edits. The identification of genome-editing conditions that
Poly (ADP-ribose)-binding promotes Exo1 damage recruitment and suppresses its nuclease activities
Cheruiyot A, et al.
DNA Repair, 35, 106-115 (2015)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 10108626001 | 04061837684432 |