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10108987001

Roche

Pyrophosphatase, inorganic (PPase)

from yeast

Synonym(s):

inorganic pyrophosphatase

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About This Item

UNSPSC Code:
12352204
EC Number:
3.6.1.1 (BRENDA, IUBMB)
Specific activity:
~200 units/mg protein (At 25 °C with inorganic pyrophosphate as the substrate.)
Biological source:
yeast
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biological source

yeast

form

suspension

specific activity

~200 units/mg protein (At 25 °C with inorganic pyrophosphate as the substrate.)

packaging

pkg of 1 mL (1 mg)

manufacturer/tradename

Roche

storage temp.

2-8°C

General description

The enzyme pyrophosphatase is ubiquitously distributed in a number of organisms.
Pyrophosphate phosphohydrolase

Application

Inorganic pyrophosphatase has been used in enzyme assays and enzyme inhibition studies.
Pyrophosphatase, inorganic (PPase) has been used in :
  • Enzymatic nitric oxide (NO) detection system
  • In vitro transcription
  • Multiply-primed rolling circle amplification

Biochem/physiol Actions

Inorganic pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate.
Mg ions are necessary for catalytic activity of crystalline pyrophosphatase.
Specific activity: Approximately 200 U/mg at +25°C with inorganic pyrophosphate as the substrate.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Analysis Note

Contaminants: <0.01% ATPase and phosphatases, each (at pH 7 and 4-nitrophenyl phosphate as the substrate).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

No data available

Flash Point (°C)

No data available

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