Product Name
Reverse Transcriptase AMV, solution, >50 units/μg protein, suitable for RT-qPCR, suitable for RT-PCR
form
solution
specific activity
>50 units/μg protein
feature
dNTPs included: no
hotstart: no
packaging
pkg of 1,000 U
manufacturer/tradename
Roche
storage condition
avoid repeated freeze/thaw cycles
parameter
42 °C optimum reaction temp.
technique(s)
RT-PCR: suitable
RT-qPCR: suitable
input
purified RNA
detection method
probe-based
Quality Level
Analysis Note
Absence of contaminants: Tested for the absence of detectable nonspecific RNases and nonspecific DNases in incubations with various nucleic acids (analyzed by gel electrophoresis) according to the current Quality Control procedures.
Function test: Reverse Transcriptase AMV is function tested in the cDNA Synthesis Kit and RT-PCR.
Function test: Reverse Transcriptase AMV is function tested in the cDNA Synthesis Kit and RT-PCR.
Application
Reverse Transcriptase AMV is suitable for:
- First- and second-strand cDNA synthesis and synthesis of first strand cDNA for use in subsequent amplification reactions (RT-PCR)
- Dideoxy DNA sequencing
- Primer extension
- RNA sequencing
- 3′-end labeling of DNA fragments
- Generation of single-stranded probes for genomic footprint experiments
Biochem/physiol Actions
AMV (Avian Myeloblastosis Virus) Reverse Transcriptase is an RNA-directed DNA polymerase and a DNA-dependent DNA polymerase. The AMV reverse transcriptase requires a primer and Mg2+ or Mn2+ for activity.
Heat inactivation: 5 min, 95 °C
Heat inactivation: 5 min, 95 °C
Features and Benefits
- Efficiently transcribes total RNA, mRNA, viral RNA and RNA rich in secondary structures
- Procure full length cDNA fragments up to 12 kb
- Higher thermostability (up to 60°C) and specificity than M-MuLV Reverse Transcriptase
General description
Reverse Transcriptase, AMV is a gene product of the RNA genome of avian myeloblastosis virus. The enzymatically active forms of the purified enzyme are α,ββ and αβ. The molecular weight of the α-subunit is 68 kDa, that of the β-subunit 92 kDa. The mature αβ form, the most active form of Reverse Transcriptase, AMV, includes a RNA-directed DNA polymerase, a DNA-dependent DNA polymerase, a RNase H, and an unwinding activity. Reverse Transcriptase, AMV is used for cDNA synthesis, for synthesis of first strand cDNA for use in subsequent amplification reactions and dideoxy DNA sequencing.
The enzyme can also be used for RNA sequencing, 3′ end labeling of DNA fragments, and the generation of ss probes for genomic footprints.
Reverse Transcriptase AMV requires a primer and Mg2+ or Mn2+ for activity.
The enzyme can also be used for RNA sequencing, 3′ end labeling of DNA fragments, and the generation of ss probes for genomic footprints.
Reverse Transcriptase AMV requires a primer and Mg2+ or Mn2+ for activity.
Other Notes
1 unit is the enzyme activity that incorporates 1 nmol of [3H]-dTMP into acid-precipitable products in 10 minutes at +37 °C with poly(A)+ · d(pT)15 as template primer.
Volume Activity: 20-25 U/μl. Please refer to the Certificate of Analysis for more information.
Volume Activity: 20-25 U/μl. Please refer to the Certificate of Analysis for more information.
For life science research only. Not for use in diagnostic procedures.
Packaging
1 kit containing 2 components
Preparation Note
Storage buffer: 200 mM potassium phosphate, 2 mM dithiothreitol, 0.2% Triton X-100 (v/v), 50% glycerol (v/v), pH 7.2.
Store at -15–-25 °C. (Storage at -70 °C is more likely to deteriorate the enzyme via repeated freezing and thawing.)
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Regulatory Information
常规特殊物品
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