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10109134001

Roche

RNase

from bovine pancreas

Synonym(s):

Rnase

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
Specific activity:
~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)
Biological source:
bovine pancreas
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biological source

bovine pancreas

form

dry powder (prepared from crystallized ribonuclease)

specific activity

~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)

packaging

pkg of 500 mg

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

General description

Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.
Crude mixture of RNases.Dry powder (prepared from crystallized ribonuclease).

Application

RNase has been used in analysis of DNA fragmentation using agarose gel electrophoresis. It has been used during flow cytometry analysis.
RNase has been used in DNA ladder assay.

Biochem/physiol Actions

Heat inactivation: 1 mM EDTA

Preparation Note

Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5.

Other Notes

For life science research only. Not for use in diagnostic procedures.
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under the assay conditions; A0 to A1 corresponds to the total conversion, A1 being the final absorbance.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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