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10127655001

Roche

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

grade I, from yeast

Synonym(s):

G6P-DH, Glucose-6-Phosephate Dehydrogenase

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About This Item

UNSPSC Code:
12352204
EC Number:
1.1.1.49 (BRENDA, IUBMB)
NACRES:
NA.54
Specific activity:
~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)
Biological source:
yeast
Concentration:
≥0.1-1.0 % (w/w)

Key Documents

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biological source

yeast

form

suspension

specific activity

~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)

packaging

pkg of 1 mL (5 mg/ml)

manufacturer/tradename

Roche

concentration

≥0.1-1.0 % (w/w)

technique(s)

activity assay: suitable

color

white to yellowish

optimum pH

9.2

solubility

water: miscible

suitability

suitable for UV spectrophotometry and general use

NCBI accession no.

CAA93357

UniProt accession no.

P11412

application(s)

life science and biopharma

foreign activity

6-PGDH <0.0100%, CK <0.00100%, GR <0.0100%, HK <0.0100%, PGI <0.00200%, PGluM <0.0100%

shipped in

wet ice

storage temp.

2-8°C

Gene Information

Saccharomyces cerevisiae ... ZWF1(855480)

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General description

Glucose-6-phosphate dehydrogenase (G6P DH) has an active dimeric form, whereas its monomeric and tetrameric forms are inactive. At low NADP+ and NADPH levels and under normal conditions, the enzyme exists as equilibrium of tetramers and dimers, whereas at high NADP+ and NADPH levels the equilibrium shifts to inactive tetramers.

Application

Component of cofactor recycling systems for NADPH.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6P-DH) is involved in the pentose phosphate pathway. It is responsible for catalysing the oxidation of glucose-6-phosphate (G6P) to gluconolactone-6-phosphate, with NADP+ simultaneously accepting hydrogen to form NADPH, thereby maintaining NADPH levels in cells.

Features and Benefits

Contaminants: <0.001% CK, <0.01% GR, HK, 6-PGDH, and PGluM each, <0.002% PGI each, <0.002% PGI
Approximately 350U/mg at +25°C with glucose-6-phosphate as the substrate.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Activator: Mg2+ (5 to 10 mM)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
48693331
11452226
11452225
11452224
11452223

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Matthew G S Norris et al.
Biochemical and biophysical research communications, 405(3), 388-392 (2011-01-18)
Enzyme kinetic parameters for rate equations are vital in metabolic network simulation, a major part of systems biology research efforts. Measurements of Michaelis-Menten kinetic parameters Km and Kcat have been performed for enzymes glucose-6-phosphate dehydrogenase (G6P DH) under crowded conditions
Chien-I Yang et al.
iScience, 25(8), 104756-104756 (2022-08-10)
The removal of the N-terminal formyl group on nascent proteins by peptide deformylase (PDF) is the most prevalent protein modification in bacteria. PDF is a critical target of antibiotic development; however, its role in bacterial physiology remains a long-standing question.
Bárbara Della Noce et al.
The Journal of biological chemistry, 298(3), 101599-101599 (2022-01-23)
Carbohydrate metabolism not only functions in supplying cellular energy but also has an important role in maintaining physiological homeostasis and in preventing oxidative damage caused by reactive oxygen species. Previously, we showed that arthropod embryonic cell lines have high tolerance
Neal J Dawson et al.
eLife, 9 (2020-07-31)
High-altitude environments require that animals meet the metabolic O2 demands for locomotion and thermogenesis in O2-thin air, but the degree to which convergent metabolic changes have arisen across independent high-altitude lineages or the speed at which such changes arise is

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