biological source
yeast
form
suspension
specific activity
~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)
packaging
pkg of 1 mL (5 mg/ml)
manufacturer/tradename
Roche
concentration
≥0.1-1.0 % (w/w)
technique(s)
activity assay: suitable
color
white to yellowish
optimum pH
9.2
solubility
water: miscible
suitability
suitable for UV spectrophotometry and general use
NCBI accession no.
UniProt accession no.
application(s)
life science and biopharma
foreign activity
6-PGDH <0.0100%, CK <0.00100%, GR <0.0100%, HK <0.0100%, PGI <0.00200%, PGluM <0.0100%
shipped in
wet ice
storage temp.
2-8°C
Gene Information
Saccharomyces cerevisiae ... ZWF1(855480)
General description
葡萄糖-6-磷酸脱氢酶(G6P DH)具有活性的二聚体形式,其单体和四聚体形式是无活性的。在低NADP+和NADPH水平且在正常条件下,酶以四聚体和二聚体形式存在并保持平衡,而在高NADP +和NADPH水平时,这种平衡转变为无活性的四聚体形式。
Application
作为NADPH的辅因子再循环系统的组分。
Biochem/physiol Actions
葡萄糖-6-磷酸脱氢酶(G6P-DH)参与戊糖磷酸途径。 它能够催化葡萄糖-6-磷酸(G6P)的氧化,产生葡糖酸内酯-6-磷酸,其中NADP+同时接受氢以形成NADPH,从而维持细胞中的NADPH水平。
Features and Benefits
污染物: <0.001% CK,<0.01% GR、HK、6-PGDH和PGluM,<0.002% PGI,<0.002% PGI
在+25℃下约350 U/mg,以葡萄糖-6-磷酸为底物。
Physical form
悬浮于3.2 M硫酸铵溶液中,pH约为6
Preparation Note
活化剂:Mg2+(5至10 mM)
Other Notes
仅用于生命科学研究。不可用于诊断。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash