产品名称
葡萄糖-6-磷酸脱氢酶（G6P-DH）, grade I, from yeast
biological source
yeast
form
suspension
specific activity
~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)
packaging
pkg of 1 mL (5 mg/ml)
manufacturer/tradename
Roche
concentration
≥0.1-1.0 % (w/w)
technique(s)
activity assay: suitable
color
white to yellowish
optimum pH
9.2
solubility
water: miscible
suitability
suitable for UV spectrophotometry and general use
NCBI accession no.
UniProt accession no.
application(s)
life science and biopharma
foreign activity
6-PGDH <0.0100%
CK <0.00100%
GR <0.0100%
HK <0.0100%
PGI <0.00200%
PGluM <0.0100%
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Gene Information
Saccharomyces cerevisiae ... ZWF1(855480)
Application
作为NADPH的辅因子再循环系统的组分。
Biochem/physiol Actions
葡萄糖-6-磷酸脱氢酶（G6P-DH）参与戊糖磷酸途径。 它能够催化葡萄糖-6-磷酸（G6P）的氧化，产生葡糖酸内酯-6-磷酸，其中NADP+同时接受氢以形成NADPH，从而维持细胞中的NADPH水平。
Features and Benefits
污染物: <0.001% CK，<0.01% GR、HK、6-PGDH和PGluM，<0.002% PGI，<0.002% PGI
在+25℃下约350 U/mg，以葡萄糖-6-磷酸为底物。
General description
葡萄糖-6-磷酸脱氢酶（G6P DH）具有活性的二聚体形式，其单体和四聚体形式是无活性的。在低NADP+和NADPH水平且在正常条件下，酶以四聚体和二聚体形式存在并保持平衡，而在高NADP +和NADPH水平时，这种平衡转变为无活性的四聚体形式。
Other Notes
仅用于生命科学研究。不可用于诊断。
Physical form
悬浮于3.2 M硫酸铵溶液中，pH约为6
Preparation Note
活化剂：Mg2+（5至10 mM）
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
此项目有
Matthew G S Norris et al.
Biochemical and biophysical research communications, 405(3), 388-392 (2011-01-18)
Enzyme kinetic parameters for rate equations are vital in metabolic network simulation, a major part of systems biology research efforts. Measurements of Michaelis-Menten kinetic parameters Km and Kcat have been performed for enzymes glucose-6-phosphate dehydrogenase (G6P DH) under crowded conditions
Bárbara Della Noce et al.
The Journal of biological chemistry, 298(3), 101599-101599 (2022-01-23)
Carbohydrate metabolism not only functions in supplying cellular energy but also has an important role in maintaining physiological homeostasis and in preventing oxidative damage caused by reactive oxygen species. Previously, we showed that arthropod embryonic cell lines have high tolerance
Chien-I Yang et al.
iScience, 25(8), 104756-104756 (2022-08-10)
The removal of the N-terminal formyl group on nascent proteins by peptide deformylase (PDF) is the most prevalent protein modification in bacteria. PDF is a critical target of antibiotic development; however, its role in bacterial physiology remains a long-standing question.
Neal J Dawson et al.
eLife, 9 (2020-07-31)
High-altitude environments require that animals meet the metabolic O2 demands for locomotion and thermogenesis in O2-thin air, but the degree to which convergent metabolic changes have arisen across independent high-altitude lineages or the speed at which such changes arise is
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